The Hydrolization Of Soy Phospholipid And The Influence Of Its Product’s Quality

Phosphatidylcholine(PC) has many important physiological functions, so it has been widely used in the fileds of food, nutrition,medicine, cosmetics and so on.Nuclear magnetic resonance(NMR) analysis of phosphatidylcholine was conducted using the internal standard methodin this research. 31P-NMR spectra were obtained with a BRUKER AV500 spectrometer at 25 ℃ in methanol-d using triphenyl phosphate as the internal standard. For each measurement, the following parameters were applied:the detection frequency 202.446 MHz, spectra width 81521.742 Hz, pulses width 11.9 μs,acquisition time 0.4 s, delay time 2 s, and the number of scan 160. The integration of signals at δ =-0.57 ppm(PC) and-17.45 ppm(triphenyl phosphate) were used for the quantification. By linear regression of these signals, a regression equation of the signal area of PC and concentration was obtained as y = 2.672 x – 0.037, R2=0.998. The precision of the instrument(RSD) is 0.68%(n = 6). The average recovery for PC is 102.50%, with an RSD of 4.26%.L-alpha-Glycerophosphorylchoine(GPC) is a kind of intermediate for metabolism of phospholipids, the complete hydrolysate from two fatty acyl in the phosphatidyl choline molecules, and meanwhile, a kind of important neurotransmitters and phospholipids precursor.In this research,organic amine was used to hydrolyzephosphatidyl choline to prepare GPC.The impactofamines types andtheir addrition level,the volume ratio of methanol to water, the reaction timeandtemperature on GPC production was investigated. The GPC rate achieved 80.54% under the following optimal conditions:butyl amine in 25% methanolin the water, the reaction time of 6 hours, the temperature of 70℃ and 2:1 of the quality ratio of the added amine and the PC.