The Design And Experimental Research Of The Glass Capillary Tension Feeding System Based On Laser Heating

At present, there are two kinds of nanopores for the DNA sequencing technology: one is the biological nanopire, and the other one is the solid-state nanopore. Compared with biological nanopres, solid-state nanopores have some significant advantages such as higher strength, higher stability and longer service life. The nanopores based on glass with good physical properties and low cost, comparing with traditional nanopores on the film, just need simple facilities, and are more suitable foe industrial application besides laboratory. The glass capillary tension system is designed and made. There are two systems based on induction heating and laser heating. Relevant experiments are made to study the change low of the glass capillary in tension.Do experiments by the system based on induction heating to study effects of tension rate and heating temperature on inner and outer diameters of the glass capillary. The results show that slower tension rate leads to smaller diameters, as well as the higher heating temperature. The inner diameter of the glass capillary stretched by this system is as small as 500 nm. There is a fibrosis problem in the stretched glass capillary. To solve this problem, the heating range must be reduced.The tension system based on laser is designed to realize the heating range reduce. The system is composed by mechanical structure and system control. Structure design mainly includes the laser heating part and the ultrasonic motor driving part. System control design includes hardware and software. The prototype is built and the primary experiments are made. Stretch glass capillary with thin pore wall directly by the system on laser heating. On orthogonal experimental design to do experiments and analyze data, and get the optimum parameters. For the glass capillary with greater wall thickness, a second-stretched experimental method is proposed. With the overall experiment with single factor, effects on the glass capillary at different duty cycle is reached. Results show that the second-stretched experimental method can be used to manufacture nanopores with smaller inner diameters, as small as 30 nm.