Promote Methods And Optimum Combination Research For Cultivation Of Microalgae Using Municipal Wastewater

Cultivating microalgae by municipal sewage, harvesting mature microalgae, refining biodiesel as a set of complete technological process, can not only ease the increasingly serious energy crisis; but also alleviate the water pollution. At present, the technology is welcomed, among them,under the premise of guaranting the microalgae oil production,how to get a great deal of mature microalgae in a ver short time, is the source of the whole process running smoothly. This to pic’s goal is to find a set of combination optimization method to maximize the growth of microalgae, in order to harvest the mature high-lipid microalgae in the shortest possible time.This can lay a solid foundation for the whole process to industrialization.Through reading lots of literatures at home or abroad and summarizing the research work our scientific research group has done before, determining the experimental microalgae: 9 chlorella pyrenoidosa by the method of N + ion implantation; Screening out the four external use stimulants: plant hormones, metal ions, bacteria and CO2 enrichment. through the experiment, the selected four promotion methods(plant hormones, metal ions, bacteria and CO2) all have a great effect on promoting the growth, dry weight and oil production of 9 chlorella pyrenoidosa.The optimum concentration are: 1.0 mg/L hormone 2, 4-D, 10-5 mol/L iron ion, 3.5 m L(109 / m L) p Hotosynthetic bacteria bacterium liquid, 10% carbon dioxide.The optimal combination which can mostly promote the growth and oil accumulation of 9 chlorella pyrenoidosa under the laboratory conditions, is 1.0 mg/L 2, 4-D, 10-7 mol/L iron ion, 4.5 m L(109 / m L) p Hotosynthetic bacteria, 8% carbon dioxide. And among the four external use stimulants, 2, 4-D and p Hotosynthetic bacteria play more important roles than iron and carbon dioxide in promting the growth and oil accumulation of 9 chlorella pyrenoidosa The optimal combination(1.0 mg/L 2, 4-D, 10-7 mol/L iron ion, 4.5 m L(109 / m L) p Hotosynthetic bacteria, 8% carbon dioxide) also can be effective in the outdoor large-scale cultivation.algae dry weight and oil production of 9 chlorella pyrenoidosa is increased. And the whole culture process operations stability, various pollutant reaches emission standards.