Aggregation Of Quantum Dots For The Detection Of Biotinylated Proteins And For The Super-resolution Microsocopy

Quantum dot is a kind of semiconductor luminescence nano-materials. Compared with conventional organic fluorescent dyes, quantum dots have many excellent properties, such as broad absorption spectra, narrow emission spectrum, high quantum yield, and anti-photobleaching. In the field of chemical analysis, biological sensing,molecular imaging QDs have been widely applied. In this thesis, we utilized the wide-field microscopy to quantify biotinylated protein and to super-localize QDs in the aggregation. The main contents are as follows:1. Measuring the average degree of aggregation(DOA) of quantum dots. We firstly developed a method to monitor the process of blue shift and bleaching of an aggregation of quantum dots. Based on the asynchronous blue shift and bleaching the DOA were determined. The QD-biotin solution was incubated with strepavidin(SA).In the range of 0 nM to 25 nM of SA, the DOA linearly increases with increasing SA concentration.2. The influence factors and the average degree of aggregation of the quantum dots used in the quantitative study of protein. This chapter explores the protein concentration, ionic strength, the impact of the buffer pH, reaction temperature,reaction time and other factors on the degree of quantum dots reunion. On the basis of the parameter optimization, for AT-Ⅲ-Biotin, examines the relationship between the average degree of quantum dots and its concentration, so establish a new methods of biotinylated protein.3.Super-resolution and factors about the aggregation of quantum dots. This chapter examines the quantum dot super-resolution localization accuracy factors, such as exposure time, gain, external forces, etc. According to blink and blue spectral shift of the quantum dots, quantum dot aggregates within the diffraction limits ofpositioning a plurality of quantum dots were initially studied the relationship between quantum dot.