Low Temperature Of Straw Strains Enzymology Properties, Degradation Effect And Its Influence On Soil Nutrient And Enzyme Activity

Straw returned is a raise the utilization ratio of straw, the effective measures to improve soil fertility, is the main way that the straw resource utilization. But due to reasons such as the temperature in the north, after the straw into the soil, the decomposing and slower, reach the desired effect. In order to make the straw under the condition of low temperature or room temperature decay quickly, improving soil fertility, and shall not affect the function of farming season, at the same time as the straw returned application of low temperature efficient fast decomposition straw bacterium agent is very necessary. Low temperature of straw degradation bacteria agent application has certain influence on soil nutrients. So, find out low temperature of straw degradation bacteria agent application influence on soil nutrient and soil enzyme activity, has important theoretical and practical significance.Based on the simulation of the straw returned the basin culture experiment and indoor analysis, the method of combining study the low temperature enzyme activity characteristics, straw returned straw degradation bacteria under the low temperature of straw degradation bacteria of straw degradation effect and the influence of soil nutrient and soil enzyme activity, to make clear straw degradation bacteria at low temperature in the condition of low temperature mechanism of straw, as to provide theoretical basis for straw bacterium agent applied in the fields.Research results show that the test of the selected A14 and room A41 two strains of bacteria agent, at 12 ℃under the condition of cultivation of 8 d, the enzyme activity reached the maximum value of bacteria agent, including A14 cellulose enzyme activity of 28.95 U/ml, and A14 and room A41 mixed strains, cellulose enzyme activity reached 36.54 U/ml. The cellulose enzyme activity, and amylase activity of bacteria agent, pectic enzyme activity and lignin enzyme activity in training in 8 d peaks, thus illustrates in the 8 d, the enzyme activity of bacteria agent is strongest. Mixed bacteria agent enzyme activity is higher, therefore in the process of degradation, degradation speed is faster than other bacteria agent.Training after 8 d, the same strain activity of different enzymes. Among them, the strain of A14 performance is: the cellulase activity > pectinase >lignin enzymes amylase; Room A41 performance for cellulose enzyme activity >amylase and pectinase enzyme > lignin and room A41 showed the same patterns; Mixture performance is: the cellulase activity > lignin enzymes pectic enzymes amylase; This shows that mixing two kinds of bacteria can increase the activity of lignin, under the condition of low temperature, promote the straw may be cellulose enzyme and the degradation of lignin enzymes.In the simulation of the straw returned pot cultivation experiment, after the cultivation of 20 d, strain A14, room A41 and mixed strains of maize straw cellulose degradation rate reached 35.10%, 34.12% and 35.10%, 13.67% ~ 18.45% higher than sterile control. Applying inoculants of maize straw decomposition rate of cellulose, hemicellulose and lignin is significantly higher than the sterile control. At the same time, combining with electron microscope scanning results showed that strains for the degradation of straw started from the inside, and then make the whole straw structure begins to appear loose condition, processing of corn straw application of mixed bacteria agent on the structure damage degree is higher.Straw returned by applying straw degradation bacterium agent to improve the soil nutrient content and soil organic matter content has a promoting effect, different treatment were significantly higher than that of not applying inoculants. Applying inoculants on the soil organic matter content can be increased by 11.64% ~ 27.46%, 29.33% ~ 44.12% higher than ck, application of straw degradation bacteria agent soil available nitrogen, phosphorus and potassium increased to some extent, the content of soil available p increased to 12.46% ~ 12.46%, especially on the increase of mixed bacteria agent degree is most obvious. Applying inoculants on the improvement of soil enzyme activity at the same time also have obvious effect, which increased soil urease is most obvious, can reach 55.88% ~ 68.21%, and applying mixed bacterium agent to the increase of the content of soil urease, the largest is 61.42% higher than that of not applying inoculants. On soil invertase, soil phosphatase and catalase, all have varying degrees of increase.