The Study Of The Interaction Between Mental And Protein By Fluorescence Spectrometry

The interaction between metal and protein has become a heated issu e in the field of life science and technology for decades. Protein is extremely important macromolecule around the biological tissues, and plays a particularly important role in the continuation of life and development. The metal palladiu m (pd) ions of entering the organism may react with protein and affect the n ormal physiological activities. And palladium complexes with high anti-cancer p roperties can be also used to treat diseases. It is a potential risk that Gold na noparticles (Au NPs) used as drug carrier in organism is worthy of further res earch. Fluorescence spectrometry has been spent on researching the interaction of the metal with protein, it is an deep understanding of the mechanism of int eraction of the metal with protein and provides useful data and information, an d then provides a theoretical guidance for metal toxicology and the developme nt of new nano drugs.In this paper, the interaction of the metal (Pd(â…¡) and gold nanoparticles) with bovine serum albumin were studied by ultraviolet absorption spectroscopy, steady-state and time-resolved fluorescence spectroscopy. The more content of each part is as below:(1) The interaction of Pd(â…¡) with aromatic amino acids(Trp, Tyr and Phe) were researched by using steady state and transient fluorescence spectroscopy. The positions of emission wavelength and changes of quenching degree were analyzed to discuss the spectral changes of features of amino acid fluorescence intensity with Pd (II) joining; the standard curves of three systems were drawed according to the relationship of â–³F and c, and then the detection limits were calculated; the absorption spectra, the quenching constant Ksv and lifetime illustrated this procedures are static quenching.(2) The interaction of Pd(â…¡) with BSA was studied by ultraviolet absorption spectroscopy, the steady-state and transient fluorescence spectroscopy. And the multi-peak gaussian fitting was used to analyse each fluorescence components of fluorescence spectroscopy, and the location of interaction of Pd(â…¡) with BSA may be at the 212th Trp residues; the absorption spectra, the quenching constant Ksv indicated this procedure of Pd(â…¡)-BSA system is static quenching. Finally based on the Pd(â…¡)-BSA fluorescence quenching system, the fluorescence recovery of the interaction of Pd(â…¡)-BSA system with thiol compounds (Cys) was researched(3) The seed crystal growth approach were used to make the controllable size Au NPs controllable size, and the PH, seed amounts and the amounts of HAuCl4 for different sizes of NPs were analyzed; fluorescence intensity of the interaction of four sizes with different concentrations of Au NPs with BSA had been measured; the quenching mechanism of gold nanoparticle-BSA system was studied, and the experiments showed that the quenching procedure of gold nanoparticle-BSA system is static quenching.