Study Of The Preparation And Properties Of PVA/CS Nanofiber Materials

In the present study, PVA/CS hybrid nanofibers were prepared by electrospinning method. We investigated the effect of the mass ratio between polyvinyl alcohol and chitosan on the morphology of fibers by scanning electron microscopy. Studies have shown that with the increase of the concentration of electrospinning solution, the diameter of fibers became bigger, and the morphology of fibers became more regular. When the concentration of solution reached a critical value, the morphology of fibers was the best and the size of fibers was uniform; above this critical value, the size distribution of fibers was not uniform. Glutaraldehyde vapor was used to crosslink the composite fibers. Through testing the contact angle of the PVA/CS composite nanofibers before and after crosslinking, the hydrophilic properties of the prepared PVA/CS composite nanofibers with different mass ratios were investigated. We found that the hydrophilic properties of the composite nanofibers after crosslinking reduced and the contact angle became larger.Polyvinyl alcohol/chitosan(PVA/CS) core-shell nanofibers are successfully fabricated by a simple coaxial electrospinning method, in which PVA forms the core layer and CS forms the shell layer. With the change of the feed ratio between PVA and CS, the surface morphology and the microstructures of the nanofibers are largely changed. The as-prepared core-shell fibers can be used as a carrier for doxorubicin(DOX) delivery. FT-IR analysis demonstrates that hydrogen bond between CS and PVA chains forms. The results of in vitro cytotoxicity test indicate that the core-shell fibers are completely biocompatible and the free DOX shows higher cytotoxicity than the DOX loaded nanofibers. The standing PVA/CS core-shell fibers remarkably promote the attachment, proliferation and spreading of human ovary cancer cells(SKOV3). Via observing by confocal laser scanning microscopy(CLSM), the DOX released from the fibers can be delivered into SKOV3 cell nucleus, which is significant for the future tumor therapy. And that, the as-prepared fibers exhibit controlled release for loaded DOX via adjusting the feed ratio between PVA and CS, and the DOX loaded nanofibers are quite effective in prohibiting the SKOV3 ovary cells attachment and proliferation, which are potential for chemotherapy of ovary cancer.