| Red Globe grape was selected as test material, this paper mainly discussed the postharvest grape using the high performance liquid chromatography(HPLC)method for the determination of sugar content and composition of the conditions, and studied the trend of grape fruit during storage of sugar at the same time by this way. In addition, the experiment also studied relative enzyme’s activity in the process of sucrose metabolism during the storage,got the change trend of sucrose metabolizing related enzymes, and analyzed the correlation between carbohydrate and related enzyme activity. Finally, the experiment also used a different kind of sulfur dioxide preservatives to deal with the grapes in the process of storage,got the change trend of soluble sugars, sucrose related enzyme activity and physiological and biochemical indexes of red Globe grapes during storage, carried out the analysis of the correlation at the same time, and found out the most suitable red Globe grapes storage preservative agent. Research results are as follows:By high performance liquid chromatography(HPLC)method for measured grape sugar content, got that the postharvest grape of high performance liquid chromatography(HPLC)method to detect the contents of sugar condition was: acetonitrile:water was 8: 2, the flow rate was 0.8mL/min, the injection volume was 20μL, column temperature was 35 ℃. Under these circumstances, the peak order of three sugar was fructose, glucose, sucrose,corresponding to the retention time was 15 min, 20 min, 33 min respectively.High performance liquid chromatography(HPLC)method for analysis of the grape sugar content showed that, the main composition of sugar in postharvest grape was fructose and glucose, but also contains a small amount of sugar, three sugar size relationship was:fructose>glucose>sucrose.Through the study of postharvest grape throughout the storage period, found that the content of fructose overall present the upward trend after first fall, glucose level rose during storage after the first showing a downward trend, the sucrose content showed a downward trend after the first rise. Fruit acid invertase activity decreased after the first upward trend;neutral invertase activity decreased after the first upward trend; sucrose synthase activitydecreased after the first rise; sucrose phosphate synthesis activity decreased after the first rise;sucrose net synthesis activity of the enzyme was positive after the first negative trend.Correlation analysis results showed that, there was a positive correlation between the grapes of fructose, glucose and acid invertase, neutral invertase activity; sucrose content and sucrose synthase, sucrose and sucrose phosphate synthase enzyme activity of synthetic net inter also had a positive correlation. Meanwhile, the sucrose content and fruit of Vc content,titratable acidity, soluble solids content were tested positive correlation; sucrose content and decay rate, drop rate of grain were tested a negative correlation; fructose and glucose content and reducing sugar content, total sugar content were positive correlation.In the process of grape fruit storage, using slow-released type preservative sulfur dioxide could effectively control the grape rot and threshing, extending the storage period;reducing the fruit respiration; effectively maintaining the fruit hardness; restraining the growth of the pathogenic bacteria; reducing sugar and other organic matter and nutrient consumption rates to keep the flavor of the fruit. In six kind of fresh-keeping agent of sulfur dioxide treatment,the treatment 5(one Red Globe Grape special plastic wrap)and 6(Red Globe Grapes 7+1 type preservatives)could be the best treatment approach as Red Globe grapes of long-term storage. |
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