Functional Ruthenium Polypyridyl Complexes Impact On The Stability Of Triplex RNA

Nucleic acid is the material basis of biological heredity and variation, plays an important role in the biological body. Its structures are varied under the influence of various internal and external factors, many studies have shown that the structure and conformation of small molecular compounds have an important regulatory role of the nucleic acid. Ruthenium complexes, due to a combination of easily constructed spatial structures spanning all three spatial dimensions and a rich photophysical repertoire,its interaction with nucleic acid is one of the highlights research in the biological inorganic chemistry.We have synthesized six different ruthenium complexes in this article, two of them containing thiophene ring, two auxiliary ligand complexes with 2,2’-bipyridine and two auxiliary ligand complexes with 1,10-phenanthroline, using spectral methods, viscosity methods and DFT calculations, and other research methods to explore with poly(U)?poly(A)*poly(U) interactions. Following are the research details:In chapter one, introduces the developing history of bioinorganic chemistry, the structure and function of nucleic acids, duplex chains and triplexe chains nucleic acids.In chapter two, synthesis of thiophene ring ruthenium complexes [Ru(bpy)2(btip)]2+(Ru1) and Ru(phen)2(btip)]2+(Ru2), using spectrum titration experiment, thermodynamics and fluid mechanics, DFT calculations and other research methods to investigated the binding properties of thiophene ring ruthenium complexes with poly(U)?poly(A)*poly(U), and its effect on the stability of nucleic acid structure and conformation transformation. Our study found that the complexes may by inserting function combined with triplexe RNA, can improve the stability of Hoogsteen base-paired, tend to slightly destabilize the Watson–Crick base paired duplex of the triplex, reflecting the two complexes binding more strongly to the Hoogsteen base-paired third strand than to the Watson–Crick base paired duplex under the present experiments.In chapter three, synthesis of two auxiliary ligand ruthenium complexes with 2,2’-bipyridine, research the two complexes with triplexe RNA interaction, the results show that both two complexes with triplexe RNA by intercalation mode, can effectively improve the stability of Hoogsteen base-paired, but the effect on the stability of Watson–Crick base paired was different, Ru3 has no effect on its stability, but the Ru4 has obvious destabilize effect to it.In chapter four, research in the two auxiliary ligand ruthenium complexes of 1,10-Phenanthroline with triplexe RNA interaction, the results show that both Ru5 and Ru6 with triplexe RNA by intercalation mode, the Ru5 can effectively improve the stability of Hoogsteen base paired but no effect on the stability of Watson–Crick base paired. The Ru6 can little improve the stability of Hoogsteen base paired, but also little destabilize the stability of Watson–Crick base paired.