Controllable Preparation Of Magnetic Targeting Isoniazid Nanocomposite Particles And Its Properties

Isoniazid (INH) is used as an anti-tuberculosis model drug at present, which have high selectivity, strong antibacterial action and effectivity for tuberculosis. Clinical management of the disease is limited and treatment effects is reduced because of drug-resistant, degradation of drugs before reaching their target site, toxic side effects of drugs. The magnetic targeted drug delivery system can be used to enhance the release time of INH, minimize its toxic side effects and reduce the dose frequency compared to traditional treatment protocols. It provides effective administration route for the treatment of tuberculosis. In this work, two kinds of nanocomposites including Fe3O4/CS/INH and Fe3O4/HBPE-CA/INH were prepared. The sizes, morphologies, structures and performance of the obtained nanocomposites were measured. The main works are as follows:(1) Magnetic Fe3O4 nanoparticles were prepared by chemical coprecipitation method, in which FeCl3·6H2O, FeSO4·7H2O and NH3·H2O were chosen as raw materials. The obtained FesO4 nanoparticles were spherical in shape, with narrow size distribution. The mean size of the Fe3O4 nanoparticles was 10 nm. The saturation magetisation valve of Fe3O4 nanoparticles was 76.5 emu/g. And the blocking temperature was 269 K. These results proved that magnetic Fe3O4 possessed the superparamagetic properties.(2) FesO4/CS/INH nanocomposites were prepared by ionic gelation method using chitosan, tripolyphosphate, INH and Fe3O4. The obtained Fe3O4/CS/INH nanocomposites were spherical in shape and the mean size of nanocomposites was 17 nm. The loading capacity of INH was 15.9% and the encapsulation efficiency of INH was 12.5%. The result of FTIR indicated an electrostatic interaction between CS, INH and Fe3O4. The saturation magetisation valve of the obtained nanocomposites was 47.5 emu/g. The blocking temperature was 270 K. These results proved that the obtained nanocomposites possessed the superparamagetic properties. And the obtained nanocomposites possessed slow-releasing property and the pH sensitivity. The result of cytotoxicity showed that the obtained nanocomposites exhibited noncytotoxicity and biocompatiblility.(3) Fe3O4/HBPE-CA/INH nanocomposites were prepared by a simply solvent evaporation method. The obtained Fe3O4/HBPE-CA/INH nanocomposites were spherical in shape and the mean size of nanocomposites was 12 nm. The loading capacity of INH was 22.8% and the encapsulation efficiency of INH was 14.8%. The result of FTIR indicated an electrostatic interaction between INH, HBPE-CA and Fe3O4. The saturation magetisation valve of the obtained nanocomposites was 61.0 emu/g. The obtained nanocomposites possessed slow-releasing property and the pH sensitivity. The result of cytotoxicity showed that the obtained nanocomposites exhibited noncytotoxicity and biocompatiblility.Therefore, two kinds of obtained nanocomposites can be used as magnetic targeted drug carrier, which have application prospect.