Synthesis Of Functionalized Magnetic Mesoporous Nanocomposites And Their Applications In Peptidome Analysis

As a branch of proteomics, peptidome has been extensively applied in biomarker discovery, early diagnosis and pharmacy. In the research of peptidome, sample pretreatment plays a vital role. At present, development of some novel methods for selective isolation and enrichment of peptides from complex biological samples has been attracted considerable attention. Magnetic nanomaterial, in virtue of the modifiable surface chemistry and magnetic responsivity, has been widely used to targeted cell separation, drug delivery, enzyme immobilization and protein separation. Focusing on this issue, in this thesis, we purposed two different methods for synthesis of magnetic mesoporous nanocomposites, which were also attempted to apply to the separation and analysis of target peptides in complex biological samples. The contents of this paper include:In chapter 1, a general review of nanomaterial and its synthetic technology, as well as the research progress of nanomaterial was introduced, specially focusing on description of magnetic nanomaterial in the synthetic approaches and the recent applications. On the other hand, the progress of proteomic research and its enrichment methods were described in detail, in particular, the application of magnetic nanomaterial in separation and enrichment of protein/peptides were comprehensively introduced In addition, the significance and content of this thesis were also briefly presented.In chapter 2, a novel "one-pot" method was developed for synthesis of hydrophilic magnetic mesoporous nanomaterial, in which Fe3O4 with-400 nm was prepared by the solvothermal method and thus glucose amidated silane, as a functional monomers, was coated at the surface of the magnetic nanoparticles by sol-gel process. The morphology and structure property of the resultant magnetic mesoporous nanocomposites were characterized by Transmission electronic microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR) and Nitrogen adsorption instrument and so on. The results showed the MMCs have a core-shell structure, large specific surface area and narrow pore size distribution. Combined with HPLC-MS technique, the practicality of the MMCs was further assayed by selective enrichment of hydrophilic target peptides from the serum and HRP digestion and the good results demonstrated its potential in peptidome analysis.In chapter 3 a novel "one-pot" method was developed for synthesis of hydrophobic magnetic mesoporous nanomaterial, in which Fe3O4 was prepared by the solvothermal method and regarded as the core and thus phenylated silane, as a functional monomers, was coated at the surface of the magnetic nanoparticles with the core-shell structure by sol-gel process. The morphology, composition and structure property of the resultant magnetic mesoporous nanomaterial were characterized by Transmission electronic microscopy (TEM), Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR) and Nitrogen adsorption instrument and so on. Combined with HPLC and HPLC-MS technique, the results showed that the mesoporous nanomaterial own doubt effects including hydrophobic effect and size exclusion.The selectivity of MMCs was evaluated by separating peptides from protein-peptide mixture. The practicality of the MMCs was further assayed by selective enrichment of hydrophobic target peptides from the serum digestion and there are good results demonstrated its potential in peptidome analysis.