Hydrogen Sulfide Removal By Paenibacillus Sp. In A Biotrickling Filter

Odor pollution is one of the prominent environmental problems during China’srapid industrialization and urbanization process. H2S, among all the malodorous gas,is the most common and dangerous sulfur-containing substance. H2S does not onlydirectly be harmful for human health and the growth of plants and animals,but alsocauses severe corrosion of devices, instrumentation and piping in oil, natural gas, coalchemical and other industrial industries. Therefore, the purification of H2S hasbecome an urgent problem in economic and environmental development that needs tobe solved. Of all the methods treating H2S, the biological method, with its advantagesof simple technology, low cost, high efficiency and environmental friendly, hasbecome a forefront study area home and abroad. In this study, we use biologicalmethods to study the purification process of H2S. The main contents of this study are:For the first time,we use Paenibacillus sp.for the application on sulfide removal.The growth curve in different media of strain Y-5were measured, and we also test theusing of carbon, nitrogen and sulfur compounds sources of strain Y-5. Test resultsshow that strain Y-5is able to grow well in LB medium; CO2can be fixed by strainY-5and used as carbon source as well.Strains Y-5can use glucose,sucrose and starchas carbon source for chemo-heterotrophic growth.Y-5can also use ammonium,nitrite,and nitrate as nitrogen source.Thiosulfate can be best used among all the sulfurcompounds.Bio-trickling filters is used to decide the best packings for the strain to grow on,results show that the ladder ring is the best for Y-5strain. A set of experiment isdesigned to make sure stain Y-5can truly treat H2S.The optimum temperature of thesystem is25℃.The study also test the removal of H2S using strain Y-5under different condition.Effects of the inlet concentration of H2S, empty bed residence time (EBRT), liquidflow rate and pH on the removal efficiency of H2S were investigated. The removalefficiency reached above90%at the inlet H2S concentration of0-800ppm, thecorresponding empty bed retention time (EBRT) of42.48s and the nutrient liquidflow rate of40L·h-1. H2S maximum capacity is72.45g· m-3· h-1; Y-5strain cantolerant a wide range of pH. The good performance of Strain Y-5in a long term operation show that strain Y-5has a good prospect for industry application.