Preparation And Properties Of Polysaccharide - Based Mixture Film

Fibroblast is main cell of wound healing and repairing during wound healing process. Excessive growth of fibroblast may lead to scar proliferation, and scar proliferation is main reason of biliary stricture. Chitosan (CS) is widely used as drug delivery carrier all over the world and has good drug release properties. Konjac glucomannan (KGM) is a straight-chain plant polysaccharides with branched-chains and has excellent mechanic properties after crosslinking. Sodium hyaluronate (SH) as an important cartilage matrix proteoglycan plays an important role in improving the biological activity of materials. So, chitosan, konjac glucomannan and sodium hyaluronate were used as raw material, through the tape casting method and freeze drying technology to prepare the CS/KGM/SH porous membrane materials. It could be used as carrier released paclitaxel slowly to inhibit scar hypertrophy.Firstly, chitosan, konjac glucomannan and sodium hyaluronate were used as raw material, glycerol was used as compliant agent, hydrochloride was used as modifier, through the tape casting method, freeze drying technology and crosslinking treatment to prepare the CS/KGM/SH crosslinked porous membrane materials. The orthogonal pilot program L16(45) was used to research the optimal preparation process of CS/KGM/SH porous membrane, and the viscosity of membrane solution, the water absorption of membrane, the thickness of the membrane, the tensile strength, the elongation at break, the apparent porosity, the degradation rate were used as comprehensive evaluation index. The optimal formula for the preparation of the CS/KGM/SH porous membrane was as follows:120ml distilled water,2ml acetic acid,2.0g chitosan,0.4g konjac glucomannan,0.3g sodium hyaluronate,0.8ml glycerol,-35℃freezing. The performance of optimal group was as follows:The average viscosity of membrane solution was10450mPa·s. The average water absorption of membrane was786%. The average thickness of the membrane was1.53mm. The average tensile strength was0.348MPa and average elongation at break was8.1%. The average apparent porosity was85%. The average degradation rate in one month was82%.XRD、IR、TG-DSC and MTT assay were applied to characterize the structure, thermal stability and cytotoxicity of CS/KGM/SH porous membranes. The results were as follows:①kThe degree of crystallinity of CS after adding KGM and SH decreased, but the degree of crystallinity of CS/KGM/SH porous membranes increased significantly after cross-linking treatment.②There are a large number of hydrogen bonding among CS, KGM in SH. Partial amino group of CS appeared N-acetylation and the acetyls peak in KGM were disappear after cross-linking treatment.③The cross-linking treatment can improve the thermal stability of the CS/KGM/SH porous membranes.④Toxicity of the CS/KGM/SH porous membranes were zero order reaction, and the CS/KGM/SH porous membranes exhibited good biological activity and cytotoxicity.1#、2#、3#drug-loaded membranes were prepared through three drug concentration of paclitaxel in alcohol solution. The drug loading of them were all more than10%and drug loading efficiency of them were all more than45%. XRD、IR and TG-DSC were applied to investigate the structure and thermal stability of2#drug-loaded membranes, releasing effects of drug was also tested by a simulation method of releasing test in vitro. The results were as follows:①Paclitaxel was absorbed physically on the pore wall of CKS porous membrane.②2#drug-loaded membranes had good thermal stability.③The cumulative release rates of paclitaxel were78%on48th hour in vitro. Release rate was rapidly slowdown.