Extraction, Isolation And Purification Of Reduced Glutathione From Waste Beer

Bioactive compound glutathion ( GSH ) has significant physiological and pharmacological functions. Besides, there are considerable amounts of GSH in yeast cells. This experiment is aimed at producing GSH using waste brewery yeast as raw material. To make reasonable use of waste brewery yeast can make good profit for breweries, improve their competition ability after participating the WTO.Our essay emphasize on enzymatic extraction of GSH and its purification technology. In this essay glutathion is extracted respectively by enzymatic methods usingβ-glucanase and helicase. GSH from extract is separated and purified through 732 cationic resin. The elution effects by using eluents of different densities are studied and compared.The results indicate that: when usingβ-glucanase method, under the condition of pH4.5, 45℃,enzyme addition amount 5%( volume-weight ratio) , reaction time of 8 hours we get the maximum GSH extraction rate of 1.584%; when uing helicase method, under the condition of pH4.5, 55℃, enzyme addition amount 0.07%(calculated on weight) and reaction time of 3 hours, we get the maximum GSH extraction rate of 1.586%. Considering cost and efficiency, we preferβ-glucanase method because the enzyme itself is cheaper and addition amount is less than that of helicase method. So extraction withβ-glucanase is more suitable for industrialization. The eluent choosen for 732 cationic resin is 0.2mol/L NaOH solution because of higher recovery rate and lower content of hetero-ions and NaOH density, which make detection of GSH using ultraviolet spectrophotometry more simplified. After purification, the recovery rate of GSH is 89.19%. and through cryodesiccation, the purity of GSH solid we get is 96.80%.