Study On The Function Of Rice Heterotrimeric G Protein β Subunit (RGB1)

In plant and mammalian, heterotrimeric G-proteins (G proteins) have been conversed as an important signal module in all eukaryotes, and thus to be implicated in a wide range of life progress.G proteins complex in plant is much simplier than its counterpart in mammals. Studies of plant G proteins have been more concentrated on the model plant Arabidopsis thaliana, and more on the G proteins a subunit (Ga). In this study, I through technical means, such as biochemistry and molecular biology, to let our laboratory has got RGB1/RNAi of transgenic plants be homozygotes identified, to analyze the phenotype. The major results are as follows:1. Through the RGB1 RNA interference transgenic rice taproot GUS histochemical analysis and hygromycin identification and expression analysis of the RGB1, we get different degrees of homozygotes, and provides information for the study on plant level.2. According to a study of homozygous plants, transgenic plants exhibited different from the wild-type phenotype:plant height and root length becoming short, seed getting smaller and shorter, and panicle length changes, the RGB1 involved in the regulation of plant growth anddevelopment of multiple.3. Transient expression of rice protoplast technique to study the cellularlocalization of the RGB1 showed RGB1 located in the cytoplasm, and congregate into distribution.4. Through the processing of stress in rice seedlings, research RGB1 respond to these external signals. Found RGB1 involved in drought signaling pathways.In summary, We purified the research materials and G protein beta subunits of rice (RGB1 function are analyzed, for the rice of G protein function and signal transduction pathways play a role in promoting.