Breeding Of Pueraria Litter From Fermentation Of Monosodium Glutamate Wastewater

Mother liquor with the concentration of monosodium glutamate wastewater with high organic matter, SO42-concentration of high, NH3-N concentration in high and low pH characteristics, not suitable for the growth of Aureobasidium pullulans. Mother liquor of monosodium glutamate wastewater by appropriate processing, the Aureobasidium pullulans to its growth, and through the compound mutation and optimization of Aureobasidium pullulans fermentation conditions so that Aureobasidium pullulans can grow in the mother liquor of monosodium glutamate wastewater and produce pullulan.First, the analysis of monosodium glutamate wastewater mother liquor composition. According to the characteristics of monosodium glutamate wastewater liquor monosodium glutamate wastewater determined the pretreatment liquor. And through the mother liquor after pretreatment of monosodium glutamate wastewater components were determined Aureobasidium pullulans fermentation liquor using monosodium glutamate wastewater feasibility of pullulan.Secondly, Aureobasidium Po as the starting strain, compound mutation by UV and nitrous acid method, respectively, of a single mutation and compound mutant strain Po polysaccharide and pigment yield. Experimental results show that the optimal conditions for UV treatment UV9min:nitrous acid mutagenesis optimal conditions for nitrite concentration0.05mmol/L,60s. Compound mutagenesis approach for the generation of UV-induced1â†'2of UV-induced generationâ†'UV Mutation3rd generationâ†'Mutation one generation of nitriteâ†'2on behalf of nitrite mutagenesis, The mutated strains are named for the N2P5, polysaccharide production reached4.38g/L, is1.66times the original strain, the fermentation liquid of yellow color, continuous passage many times, its yield and no significant change in character.Finally, single-factor test, a central composite design and response surface (RSM) analysis of high-yielding mutant strain N2P5determine the optimum fermentation conditions. Monosodium glutamate wastewater through pretreatment and fermentation mother liquor after the extraction of polysaccharides, COD and BOD5removal rate reached78.02%,78.36%.