Discontinuous Electric Field Force Directs The Current Characteristics Of DNA Molecules Crossing Micron Pipes

Nowadays, in researches of biomolecule chips and sensors, the electric fields is usually employed to manipulate DNA molecules transporting along micro/nano-fluidic channels, thus it has an important practical value and scientific significance for the designation of micro/nano-fluidics chips and sensers that research the current properties of DNA molecule in microchannel. In order to accurately and flexibly induce and control the DNA molecules moving within these channels, the current changes rule of buffer caused by applied bias is fundamental. The whole work can be described by following three aspects:(1) In this report, get real-time data firstly and research the rule of current changed value in microchannels systematically and quantitatively by different DNA buffer solution e.g., KCl, TE, and TBE, and the inner diameter of microchannels are5μm and10μm. The applied bias are OmV,50mV and100mV, the inner diameter of the microchannel are5μm and10μm. The results revealed that the current changed value increased with the enhanced bias voltage, pause time and concentration etc., and the recovery time is increased with the increasing applied bias and pause time. These results are the foundation of the following research of DNA molecule in buffer solution.(2) Research the current properties of buffer with DNA molecules that combine with experiments and theories.The buffers are TE and TBE buffer, and the inner diameter of experimental microchannel are3μm,5μm and10μm, and the rest experimental condition is as same as pure solution. The experimental results show that the current change value is increased with the increasing applied bias, and decreased with the increasing pause time and pH value. Meanwhile the recovery time is in direct proportion with pause time and is inversely proportional to applied bias.(3) Randomness of the current change value is increased with the increasing inner diameter of microchannel. These experimental results can be used to guide the operation for single DNA molecule moving in bio-chip and bio-senser.