Genetic Detection And Social Behavior Of Closed Population Of Brandt 's Voles

The Brandt’s vole (Lasiopodomys branttii) as a typical rodent prairie harmful vole, has complex social behavior and is a potential animal model of social behavior and morbid behavior. There are some studies focused on the physiology, biochemistry, immunology and genetic structure of the Brandt’s vole in common laboratory populations. In our laboratory, we have already studied the reproduction, physiology, biochemistry and histology of the Brandt’s vole in the closed colony group.4-month-old of male Brandt’s voles were chosen to detect sperm motility, the results showed that the sperm motility of the closed of Brandt’s voles depended onseasonal change, higher in summer and autume and lower in spring and winter.We used SSR marker to study the stability of genetic structure of three successive generations of the Brand’s vole in the closed colony group. Genomic DNA was extracted from tail tip following high concentration salt precipitation methods. PCR products were detection by Sequencer and access to raw data (fsa file). By the analysis of the average heterozygosity and PIC values, Brandt’s vole populations maintained high and stable genetic polymorphism in the three generations.We chose ten Brandt’s voles and BALB/c mice at 2 months of age in both genders and used elevated plus maze test and open field test to analyze the anxiety trails. The results showed the level of anxiety was higher in the Brandt’s voles than BALB/c mice. Three-chamber-box was used to compare social recognition behaviors and partner preference between the Brandt’s voles and BALB/c mice.The results indicated thatthe ability of social and connubial recoginition was higher in the Brandt’s voles. Furthermore, sucrose preference test was also used to compare the sucrose preference between the Brandt’s voles and BALB/c mice. The results showed that the sucrose preference was higher in the Brandt’s voles.The present study proved that the reproductive characteristic and genetic stability of the Brandt’s voles in the closed colony group in our laboratory, and showed that the Brandt’s voles have more social behaviors than BALB/c mice. This conclusions provided reference for the introduction and breeding of wild type voles, expanded the species of laboratory animals, and enriched the species of the animal models of social behavior.