Studies Baiduoning Emulsion Antitumor Effect

In order to sift out the best proportion of brucea javanica oil and semen cocis oil, S₁₈₀ stereo-tumor and ascites tumor-bearing mice were used as experiment objects in the study. And the best prescription, which has potential anticancer activity is named Baiduoning emulsion（BDN）. Then using S₁₈₀ stereo-tumor-bearing mice, EAC-bearing mice and Lewis-bearing mice to observe BDN’s anti-tumor effect and if it can increase Cytoxan（CTX）’s anti-tumor effect, reduce CTX’s and Fluorouracil（5-Fu）’s toxicity in normal mice. In addition index of spleen, index of thymus gland, the phagocytic activities of the peritoneal macrophages, T and B lymphocyte proliferation test（evaluated by MTT assay）were performed to observe BDN’s effects on the S₁₈₀-bearing mice, For the sake of indicating it’s anti-tumor mechanisms.Results of experiments show that, First, the best proportion of brucea javanica oil and semen cocis oil is 3:1. Second, BDN 1 and 2 g·kg^-1 i.v. markedly exerted significant inhibition on S₁₈₀ stereo-tumor, and it had better effect on tumor growth inhibition than brucea javanica oil and semen cocis oil at the same dosages; While in the survival time experiment, BDN could prolong obviously the survival time of mice treated with EAC, though the effect was lower than semen cocis oil, it was higher than brucea javanica oil. BDN 1 and 2 g·kg^-1 i.g. markedly exerted significant inhibition on Lewis, and it had better effect on tumor growth inhibition than brucea javanica oil and semen cocis oil at the same dosages. The therapeutic effect of intravenous injection was better than intragastric administration. Moreover, it could reduce toxicities which were induced by CTX or 5-Fu and strength anti-tumor effect of CTX. Third, BDN could increase the index of spleen, promote the carbon cleared index K and the phagocytic coefficientα, enhanced lymphocyte proliferation significantly in S₁₈₀-bearing mice（alone or with ConA/LPS）. No obvious influence on the index of thymus was observed.