Ghrelin Foodborne Obese Mice Effect Of Angiogenesis Markers

Objective:In this study, we investigated the effect of ghrelin administration on serum biomarkers of angiogenesis including leptin, nitric oxide (NO), vascular endothelial growth factor (VEGF), and its soluble receptor (VEGF receptor1or sFlt-1) in control-and diet-induced obese mice.Methods:Male C57BL/6mice were randomly divided into four groups, normal diet (ND) or control, ND+ghrelin, high-fat-diet (HFD) or obese and HFD+ghrelin (n=6/group). Obese and control groups received either HFD or ND for15weeks. Then, the ghrelin was injected subcutaneously100μg/kg twice daily for10days. At the end of experiment, blood samples were collected for blood glucose, serum insulin, VEGF, sFlt-1, NO, and leptin measurements.Results:The obese animals had higher serum NO and leptin concentrations (t=4.08, P<0.01; t=12.49, P<0.01) without changes in serum VEGF and sFlt-1levels (t=0.983, P>0.05; t=1.75, P>0.05) compared to control. Administration of ghrelin significantly increased serum VEGF (t=3.94, P<0.01) and decreased serum leptin (t=3.51, P<0.01) and NO concentrations (t=3.25, P<0.01) in HFD group.Conclusion:Since ghrelin changes serum biomarkers of angiogenesis.