| Objective To obtain a highly metastasis Rat tongue squamous cell carcinoma cell lines, and to lay the foundation for the establishment of highly metastatic tongue cancer animal model.Methods Different metastasis potential cell clone were isolation by improved invasion assay. Cell clone was detected by MTT assay and Cloning formation assay. Cell invasiveness and migration ability were analyzed by invasion assay and migration assay. Cell cycle time was detected by flow cytometry. The enhancer of Zeste homolog2(EZH2) protein and mRNA expression were detected by Western Blot and Real-time PCR.Results The high and low metastatic potential cells(Rca-TH、Rca-TL) were isolated. Cell colony formation migration、invasion、cell cycle、EZH2protein and mRNA expression of two groups had a statistically significant (t=3.423～59.300, P<0.05-0.001)Conclusion Two different metastatic potential cell subclones were isolated, Biological characteristics between the Rca-TH and Rca-TL had significant difference. |
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