Human Papillomavirus Establish Clinical Novel Detection Methods

Human papillomavirus (HPV) have been associated with genital warts, some oropharyngeal tumors, and particularly related to cervical cancer. Human epidermal and mucosal tissues are generally susceptible to this virus, and almost all patients with cervical cancer are infected by HPV with different degrees. Therefore, the HPV genotyping method has significant value in research for early clinical diagnosis of cervical cancer.There are around100types of HPV, with different variations in their genetic and oncogenic potential. HPV have been divided into high-risk (HR) and low-risk (LR) types according to epidemiology. The most common HR-HPV which cause cervical cancer are HPV-16,-18,-31,-33,-35,-45,-52and-58. LR-HPV, principally-6and-11, are predominantly involved in the development of genital warts. Herein, two methods have been established for detecting and typing the common HPV (-6,-11,-16,-18,-31,-33,-45,-52and-58). The methods are based on PCR-immunochromatography detection and loop-mediated isothermal amplification (LAMP) genotyping assay respectively. The assays have been further evaluated with clinical specimens that have been detected with commercialized HPV genotyping kit.Compared with traditional methods, our detection methods have advantages of rapid, sensitive, high specificity and low cost. Using crude extract of viral nucleic acid from the obtained clinical samples, the preliminary screening for all samples can be accomplished by PCR-immunochromatography method. Then the positive samples can be detected by LAMP technology. This method is superior to other HPV genotyping methods which have been commercialized.The whole process is completed within3.5hours. This rapid diagnosis system has potentical applications and will play an important role in the early prevention of cervical cancer.