Different Points Of Electroacupuncture On Serum Inflammatory Factors Cerebral Ischemia-reperfusion Rats Change

Objectives:The subject observed the the dynamic change of stress-damage-repair signal chain in the peripheral blood serum after acupuncture intervention on cerebral ischemia and reperfusion model rats. To explore acupuncture treatment’s precise mechanisms in cerebral ischemia and reperfusion inflammatory injury. To provide the esact laboratory evidence for clinical treatment programs.Methods:1, The rats were randomly divided into the control groupand model control group,10animals of each group. The model animal were evaluated by Neurological score, TTC staining(to observe cerebral infarction), and HE staining(to observe the morphological changes of the brain tissue).2, The rats were randomly divided into the control group (10animals)and model group, model animals were divided into model control group,acupuncture treatment group1(DU20, HN3and DU26), acupuncture treatmentgroup2(DU20,ST36) according to the random number table, each group was divided into6schedule groups-12h,24h,48h,72h,96h,144h,10animals of each schedule. Chose ELISA method to detect the inflammatory response related signals in serum, including stress signal-ACTH, endogenous danger signal-HSP70, start-up and adjustment signals-IL-6, IL-1β, signal of damage effect-TNFα.Results:1, Model EvaluationThe TTC staining found that there was typical dyeing missing in local ischemic brain tissue, appear white, in line with the disposable area of the middle cerebral artery,and the infarction was relatively stable before and after the optic chiasm.HE staining were observed that, staining shallow in the central farction area, decrease of the cells number, nuclear condensation, smaller and irregular of the nucleolus; interstitial edema; endothelial cell swelling; a large number of grid-like vacuole.2, The influence of different acupoints to acupuncture treatment to inflammatory response signals in serum:The inflammatory response signals ACTH, IL-1β, IL-6, TNF-α, Hsp70, in cerebral ischemia and reperfusion model rats’ serum, showed typical bimodal curve mode in the process of damage development, and the trend were similar. ACTH reached the first peak at12h, and reached the second peak at48h; IL-1β, IL-6, TNF-α, Hsp70reached the first peak at24h, an second peak-to-peak at72-96h.Electro-acupuncture treatment on Baihui, Yintang and Renzhong(DU20, HN3and DU26)could significantly lowered the the first peak’expression levels of inflammatory response signals ACTH, IL-1β, IL-6, TNF-a, and Hsp70in peripheral serum; could significantly lowered the second peak’expression levels of ACTH, IL-1β, TNF-α; could increase the second peak’expression level of IL-6.Electro-acupuncture treatment on Baihui, Zusanli(DU20,ST36) contrast the points Baihui, Yintang and Renzhong(DU20, HN3and DU26), could significantly lower the inflammatory response signals’expression levels.Conclusions:1, The brain ischemia reperfusion rat model was stable; cerebral ischemia-reperfusion injury activated the inflammatory/immune-related stress-damage-repair signal chain in body part and the whole, including peripheral serum, bilateral brain tissue; this model was copied successfully.2, Electro-acupuncture treatment on Baihui, Yintang and Renzhong (DU20, HN3and DU26) played the network regulation on stress-damage-repair signal chain, had the overall impact to the inflammatory response signals in he peripheral serum and bilateral brain tissue, including stress signal, endogenous danger signal, start and regulate signals, damage effect signals; electro-acupuncture may reduce ischemia-reperfusion injury through inhibiting excessive stress, reducing the inflammatory injury, promoting inter-cell contact, starting the repair function in advance, adjusting the healthside brain tissue to compensatory.3, Electro-acupuncture treatment on Baihui, Zusanli(DU20,ST36), contrast the points Baihui, Yintang and Renzhong, had better influences in regulating the body’s inflammatory stress response to injury, reducing the inflammatory response and inflammatory, activating repair function; combined use of acupoints on the head and body may be better in reducing inflammatory injury than using the head acupuncture points only.