| Objective:In this study, the author used Chinese medicine which activating blood、eliminating abdominal mass and killing the embryo (by the method of activating blood, eliminating abdominal mass and killing the embryo) to interference the model of pregnant rats, in order to explore traditional Chinese medicine(TCM)’mechanism from observing the cell morphology and function of trophoblast, and indirectly provided a scientific basis for the treatment of ectopic pregnancy by using TCM.Methods:The successful modeling of early pregnancy rats were randomly divided into four groups:Chinese medicine group (group A:activate blood、killing embryo and eliminate abdominal mass), western medicine group (group B: methotrexate), the combined with Chinese and western medicine group (group C:invigorate the Chinese medicine and methotrexate), the blank group (group D), Respectively give the corresponding drugs, blank group on behalf of normal saline. observed and recorded the Animals’signs daily and weighted them every two days in experimental period. Rats were killed after the end of the administration, taking femoral arterial anatomy、 anatomical pregnant mouse, ake the pregnant uterus, record the number of embryo implantation and the maximum diameter of the pregnant uterus, using ELISA measurement of serum β-HCG values, paraffin sections observed trophoblastic histopathological changes, transmission electron microscopy detection observed trophoblastic ultrastructural changes.Results:1. Impact on body weight of pregnant rat:group A rats gestational weight is in a straight line rising, gain no difference compared with group D(P>0.05). group C rats’trend were significantly after falling to rise again, the gain weight is less than the other three groups, statistically more significant difference (P<0.01).2. Impact on the number of embryo implantation:group A had no difference compared with the other three groups (P>0.05). Influences the gravid uterus:according to the average rank order by order is:group D, group A, group B, group D, after statistics processing, the other three groups, respectively, compared with the blank group had significant difference (P<0.01). 3. Impact on trophoblast cell morphology:intercellular space of group A was increased, arranged disorder, inflammatory cell infiltration, normal embryo sac form disappeared; Transmission electron microscopy (sem):a large number of chromatin in the nuclei, mitochondria swelling, part of mitochondrial crest disorders or even disappear. Group C s trophoblastic damage is the most significant in the four groups of pregnant rats:the nucleus heterochromatin increased, edge set, a crumb, dense cytoplasm, small vacuoles in cytoplasm and organelles decreases. Pathology score: group D、group B、group A、group Care:4.0,2.2,1.9,2.2, after statistics processing, the other three groups were compared with the blank group had significant difference (P<0.01), group C compared with group A and group B has significant difference (P<0.01), group A and group B has no difference (P>0.05).4. Blood β-HCG levels change:group D、group B、group A、group C’s average β-HCG serum (mIU/ml), respectively:16.6,9.08,8.55,9.08, group B、group A compared with group D had difference (P<0.05), group A and group B has no difference (P>0.05), group C combined with group D、 group B、group A had significant difference (P<0.01).Conclusion:1. Mechanism of Chinese medicine which activates blood、kills embryo and eliminates abdominal mass may change the ultrastructure of the trophoblastic affect the the trophoblastic number, morphology, secretion, synthesis, and other physiological and metabolic function, serum β-HCG levels and promote inflammation cell infiltration, leading to stagnation and demise of embryonic tissue’s development, kill the embryo and thus play a role. Trophoblast and embryo damage.2. Combine using of TCM and methotrexate made more damage to embryo and trophoblast cells than Chinese medicine or western medicine used alone, indirectly hints that it can shorten the time needed for killing embryos; At the same time the pregnant rat diarrhea incidence was lower than western medicine group. |
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