The Establishment Of Primary Lung Tumors In Nude Mice Model

Background and objective To establish a series of patient-derived pulmonary malignant tumour xenografts model in nude mice as a platform of cancer research and to observe the biological characteristics of xenografts.Methods Surgically resected pulmonary cancer specimens were implanted in the subcutaneous layer of the nude mice. The subcutaneous carcinoma was surgically removed when it grew to approximately1-2cm3cm in volume, and then re-transplanted into new nude mice. The growth process and histological property of transplanted tumor was observed.Results107cases were conducted for nude mice transplantation in total.93were later histologically confirmed as primary non-small cell lung cancer (NSCLC); among them27patient-derived lung cancer xenografts have been established successfully. By the end of the third month after transplantation, the take rate of xenografts was29%for NSCLC. Histopathology consistency was preserved among generations of xenografts.3cases of pulmonary metastatic tumor were also established. One case with an endometrium origin was used in anticancer drug activity test in vivo. Paclitaxel, Cisplatin, and Gemcitabine demonstrated varying degrees of suppression effect on xenograft growth.Conclusion The xenotransplantation models were successfully established for patient-derived pulmonary cancer in immune deficient mice. This model system displayed the biological characteristics of their human origins, suggesting that it may provide a stable, reliable, and useful animal model in human cancer research.