| "Reinforcing and reducing, adjust the balance "principle of treatment, will havethe effect of qi and yin, kidney and spleen, lung and stomach, circulation networkexperience side Huang Di Ji Xian Tang used in clinical type2diabetes treatmentexperience by Professor Ning ya gong."reinforcing-reducing the same facilities andadjust the balance "therapy for the treatment of type2diabetes in effect, to providenew ideas and methods for the clinical treatment of diabetes. In addition to theexperimental study of type2diabetic rats provide experimental basis, in order tofurther explore the mechanism of action of the "reinforcing and reducing the samefacilities, adjust the balance "therapy.The first part of the reinforcing-reducing measures and adjustthe balance on Type2diabetes islet cells to repairthe clinical observationObjective: To investigate the role of "reinforcing and reducing the same measuresto adjust the balance "therapy for type2diabetes beta cells to repair, to find effectivemethods for the clinical treatment of type2diabetes.Methods: type2diabetes in60patients met the inclusion criteria, using a simplerandom method subjects were divided into two groups, the control group andtreatment group,30cases in each group. Based on the same diet and exerciseguidance, the control group received oral metformin therapy, the treatment groupwas treated with Huang Di Ji Xian Tang. The two groups were observed for12weeks. The two groups were measured before and after treatment, with fastingplasma glucose (FPG), fasting insulin (FINS), fasting C-peptide, weight, body massindex, homeostasis model assessment of insulin resistance index, homeostasis modelassessment beta-cell function index, liver and kidney function, safety index. And Thestudy collected the clinical symptoms of the corresponding period for credit. Thestudy analysised of the results with SPSS18.0statistical, to carry out results beforeand after the control and control groups, and analysised of the biochemical changes and significance of the study and improvement of clinical symptoms.Results:(1) Before treatment, the general comparison of two groups of gender,age, duration of disease, body weight, body mass index, FPG, blood pressure was notstatistically significant (P>0.05), we can see two sets of data are comparable.(2)After treatment, the two groups of the patient’s weight, body mass index havesignificantly lower (P <0.01), the two groups were not statistically significant (P>0.05).(3) After treatment, fasting blood glucose was significantly lower (P <0.01),compared to no significant differences (P>0.05) between the two groups.(4) aftertreatment compared with before treatment, both groups significantly reduced fastinginsulin levels (P <0.01), and significantly higher fasting C-peptide levels (P <0.01).Between the two groups showed no significant difference (P>0.05).(5) aftertreatment, the two groups of HOMA-IR, ISI and before treatment were compared toa significant decrease was statistically significant (P <0.01). After treatment betweenthe two groups was not statistically significant (P>0.05). After treatment, the twogroups HOMA-β compared with before treatment were significantly increased, therewas statistically significant (P <0.01) after treatment between the two groups was notstatistically significant (P>0.05).(6) After treatment, the two groups in ALT, AST,R-GT and other indicators of liver function compared with the previous were lower(P <0.05) between the two groups only r-GT difference (P <0.05), ALT, AST nosignificant difference between the two groups (P>0.05). After treatment, the twogroups in BUN, CR had no significant change (P>0.05), no significant difference(P>0.05) between the two groups.(7) After the treatment, the two sets of symptomscores were significantly reduced compared with before treatment were statisticallysignificant differences (two groups both P <0.01). Between the two groups, thetreatment group than the control group, a statistically significant (P <0.01).(8) aftertreatment, Comparison of clinical efficacy in the treatment group efficiency efficientthan the control group, the difference between the groups was statistically significant(P <0.05).Conclusion: Huang Di Ji Xian Tang, can improve the of TCM clinical symptomsand modification of type2diabetes islet beta cell function, suggesting that the mechanism may be associated with lower blood sugar, improve insulin resistance,reduce islet cell apoptosis, repair islet cells. Reinforcing and reducing the samefacilities, adjust the balance "therapy under the guidance of Huang Di Ji Xian Tangcan reduce blood glucose, glycosylated hemoglobin metformin close, and the clinicalsymptoms effective than the control group. The second part of the reinforcing-reducing measures andadjust thebalance method with type2diabetes isletcells repair experimental studyObjective:"reinforcing and reducing the same facilities, adjust the balance"principle of treatment under the guidance of Huang Di Ji Xian Tang rat pancreaticislet cells repair, explore the Huang Di Ji Xian Tang treatment and reduction of isletcell apoptosis target of mechanism of action, and scientific reveal the mechanism ofaction of Huang Di Ji Xian Tang. Confirmed that Huang Ji Xian Tang improve isletcell apoptosis and repair islet cells in the laboratory and clinical.Methods: SPF level60male SD rats.10to set the normal group, more than50high-sugar and high-fat diet plus a one-time small dose of intraperitoneal injection ofSTZ+mental stress method induced rat model of type2diabetes. After themodeling were randomly divided into model group, high dose group, in traditionalChinese medicine dose group and low dose group, metformin group10. Each groupafter8weeks of administration determination of weight, detection FPG; takepancreatic tissues HE staining and immunohistochemistry staining.Results:(1) rat general: normal rats generally best hair shiny, activities freely,good mental state, diet, drinking water and urine normal, no death; model group, theworst messy hair dull, sluggish mental state, slow, and the emergence of thephenomenon of polydipsia and polyuria. Other drug groups within the period of timeafter the successful model of the above, the model group were improved aftertreatment by the administration.(2) weight: the end of the experiment, six groups ofthe body weight of rats significant differences were statistically significant (P <0.01). Further multiple comparisons, the normal group compared with the other five groupswere statistically significant difference (P <0.01), no significant difference (P>0.05)among the other groups.(3) serological markers: the end of the experiment, sixgroups of blood glucose was no significant difference (P>0.05).(4) HE stainingshowed that the rats in each group islet morphology: normal rats islet group cords,were round, abundant cytoplasm, form a complete, the nucleus centered, clear cellborders. Compared with the normal group, model group, the number of rat isletssignificantly reduced cell volume, different forms, scattered distribution, unevenedges, unclear boundary between the cell and the nucleus was eccentric distribution.Compared with model group, in the high group and metformin group isletmorphology significantly improved. In the group with low group did not improvesignificantly.(5) insulin immunohistochemical staining results: positive signalperformance of the cells were stained brown particles. Compared with normal group,model group, positive cells decreased significantly, different degrees of improvementin the treatment group compared with the model group.(6) six groups of ratpancreatic tissue ki67expression index of less than1%, comparatively speaking,relatively more normal group, metformin group and high ki67expression.Conclusion: This study successfully induced rat model of type2diabetes,modeling a success rate of90%. HE staining and insulin immunohistochemicalstaining by the Chinese and Western medicine intervention, Chinese high-dose group,the diabetic rat pancreatic islet cells of the biguanide group some improvement,especially islet cells secrete insulin function to some extent repair. ki67immunohistochemical staining showed that diabetes is not a tumor lesions, the extentand number of cell proliferation in the normal range. |
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