Red-billed Leiothrix Brood Morphology Of Duodenum And Ileum Tissue

The duodenums and ileums of five leiothrix lutea at1,5and9days of age were obtained, fixed in paraformaldehyde and made for paraffin sections, respectively. By histological and histochemical stain, the sections were observed by microscope. The results were showed:The histological structures of duodenum and ileum of leiothrix lutea were similar to those of poultry. Through the determination on the length of intestinal villus, crypt and the thickness of muscular layer, it was showed that the length of duodenum and crypt, with the increased age, dramatically increased, while the thickness of muscular layer in duodenum increased a little. In addition, the length of intestinal villus and crypt, and the thickness of muscular layer in ileum were sharply increased.The results showed by AB/PAS staining:There were a few goblet cells, which were mainly observed in epithelium mucosae of duodenum, while a few cells were in brunner’s gland. With the increased days of age, the number of goblet cells in intestinal villus and brunner’s gland was increased, especially in intestinal villus. There were many goblet cells in ileum, which were mainly observed in epithelium mucosae of ileum villus and enteraden. By the age increasing, the number of goblet cells in epithelium mucosae of ileum villus and enteraden was increased. By determination on IOD, it was showed that the content of acidic mucins secreting by goblet cells, with increased days of age, was increased, while the contents of neutral and mixed mucins were decreased. For ileum, the contents of acidic, neutral and mixed mucins were sharply increased.The results showed by Girmelisu staining:There were few endocrine cells in duodenum and ileum of leiothrix lutea, and the number was stable with the age increasing. Accidently, the endocrine cells were observed in the area between epithelium mucosae of intestinal villus.