MSTN Gene Mutation In Mice Immunized Impact Stress Muscle Injury

In order to investigate the effects of Myostatin mutant gene immunization on stress-induced muscular atrophy,an experiment model was established by injecting a high dose of glucocorticoids with mice. The study was carried out by two experiments. In the first experiment, twelve mice about230g were randomly divided into two groups:control group, dexamethasone(Dex) group. They were respectively treated with0.9%NaCl and dexamethasone. After the continuous intraperitoneal injection lasting for a week, the sample and the body weight were taken and the changes of microstructure in skeletal were observeed. In the second experiment, twenty four mice with similar initial weight about13g were randomly divided into three groups:control group, Dex group, Dex+MSTN mutant gene immunization group. The third group was immuned four times with MSTN mutant gene. After a week from the last immunization,the first group was treated with0.9%NaCl by he continuous intraperitoneal injection and the other two test groups with dexamethasone. The experiment lasted for a week, then the sample and the body weight were taken, the changes of microstructure in skeletal were observeed and the biochemical parameters in serum(including total protein, total amino acid and urea nitrogen)were determined. The results showed that:The dexamtheasone induced skeletal muscle atrophy with the body weight down,myocyte diminution and serious myofiber degradation,and significantly decreased the contents of total protein and increased the total amino acid and urea nitrogen in serum. However, the immunization of Myostatin mutant gene obivously weakened the effects of dexamtheasone-induced muscular atrophy which tended to improve the abnormal biochemical parameters in serum.The test will lay a foundation for researching the key locus of Myostatin gene, and provide a basis for discussing the way to improve the animal production property.