Research MONASCUS Monacolin K And Liquid Fermentation Of Monascus Pigment

Monascus ruber GM011 was used for production of Monacolin K and Monascus pigments in this study, and some effect factors have been carried out on it, such as heat shock, ultrasonic treatment, and so on.As reagents, which can change the cell membrane permeability, toluene, aether and tween-80, were added in the process of liquid fermentation of GM011. The results showed that toluene not only have inhibitory effects on the growth and the production of Monacolin K and red pigment, but also have great impact on the morphology of GM011. Tween-80 can promote the growth and the production of red pigment, but the concentration of Monacolin K in the broth is very low. Aether almost do not have impacts on the growth, the production of Monacolin K and red pigment and morphology of GM011, but have great promotion for the production of Monacolin K. When 1 ml of aether was added to 100 ml broth per 24 hours, the production of Monacolin K is up to 425mg/L, as 2.53 times as the control.Heat shock and ultrasonic treatment also have been used on the liquid cultivation of GM011. But the side effect of heat shock was raised on the metabolite of red pigments and Monacolin K, when the treating time is up to 60 minutes, the red pigment value and concentration of Monacolin K were 28% and 15% as the control respectively. Ultrasonic treatment is a good method to improve the production of red pigments and Monacolin K, which improve 22% and 94.6 % contrast to the control when the treated time is 3 minutes according to the experiment, and the dried weight of biomass had no significant change.KCL and NaNO₃ were used as osmotic stress regulator to carry out experiment. The result shows that GM011 can produce more Monacolin K when it under the osmotic stress. And it is identifed that glycerol can be instead in some extent by regulating the osmotic stress of fermentation base.The experiments of addition of power of yam and hull of orange show that the addition of yam can stimulate the production of Monacolin K. It is presumed that some components which can stimulate the production Of Monacolin K may be contained in yam. When Monaseus pigment was added into the broth, the yield of Monacolin K and the Monascus pigments were reduced. It seems that Monascus pigment may exist feedback inhibition to the common way of the synthesize of Monacolin K and Monascus pigment.In order to obtain the high production of Monacolin K, The concentration of Monacoin K in broth as the fit function, and the fermentation base components were divided into 10 factors, 50 levels using Genetic Algorithm. After four generation optimization, the max concentration of Monacolin K in the broth reached 1300.4mg/L.