Qinling Region Four Kinds Schisandra RAPD And ISSR Analysis Of Genetic Diversity

Schisandra Michx. is a perennial, woody and dioecious liane and is subordinate to Schisandraceae and is mainly distributed in the regions through southwest China to eastern China. Domestic research is limited to lignans composition analysis, classification and systematics on Schisandra Michx. plants.In this paper, four species of Schisandra Michx. plants in Qinling Mountains-Schisandra sphenanthera, Schisandra rubriflora, Schisandra grandiflora and Schisandra propinqua var. sinensis, were taken as objects to analyze the genetic diversity by using RAPD and ISSR molecular markers and thus provided experimental basis for the research of the genetic diversity of Schisandra Michx. plants and the sustainable use of medical resources. The main conclusions are as follows:1.11RAPD primers were used in DNA amplification of the four species of Schisandra Michx. plants in Qinling Mountains-Schisandra sphenanthera, Schisandra rubriflora, Schisandra grandiflora and Schisandra propinqua var. sinensis.146distinguished DNA bands totally were detected in the result of11RAPD primers, of which the number of bands of each varied from11to17, with an average of13.3bands were amplified for each primer.2. In the four species of Schisandra Michx. plants in Qinling Mountains, the percentage of polymorphic bands (PPB) in species level was55.48%～97.26%, the Nei’s genetic diversity index (H) was0.1813～0.3783and the Shannon’s diversity index (I) was0.2742～0.5555, the effective number of alleles (Ne) average (1.5172) accounted for the observed number of alleles (Na) average (1.8065)83.99%. Comparison of the genetic diversity parameters of Schisandra Michx. plants in Qinling Mountains suggests that Schisandra sphenanthera has the highest genetic diversity while Schisandra grandiflora has the lowest genetic diversity. The coefficient of genetic differentiation (Gst=0.2889) reveals that the genetic variation of the four species of Schisandra Michx. plants in Qinling Mountains was mainly intraspecific genetic variation, accounting for71.11%.3. Four species of Schisandra Michx. plants in Qinling Mountains can be divided into two categories according to the UPGMA clustering graph based on the genetic similarity coefficient of0.56by RAPD. The first type includes Schisandra sphenanthera, Schisandra grandiflora and Schisandra propinqua var. sinensis and the second includes Schisandra rubriflora while the genetic similarity coefficient of0.63four species of Schisandra Michx. plants can be divided into four categories.4.11ISSR primers were used in DNA amplification of the four species of Schisandra Michx. plants in Qinling Mountains-Schisandra sphenanthera, Schisandra rubriflora, Schisandra grandiflora and Schisandra propinqua var. sinensis.145distinguished DNA bands totally were detected in the result of11ISSR primers, of which the number of bands of each varied from12to16, with an average of13.2bands were amplified for each primer.5. In the four species of Schisandra Michx. plants in Qinling Mountains, the percentage of polymorphic bands (PPB) in species level was58.62%～93.79%, the Nei’s genetic diversity index (H) was0.2199～0.4017and the Shannon’s diversity index (Ⅰ) was0.3244～0.5772, the effective number of alleles (Ne) average (1.5452) accounted for the observed number of alleles (Na) average (1.7465)88.47%. Comparison of the genetic diversity parameters of Schisandra Michx. plants in Qinling Mountains suggests that Schisandra sphenanthera has the highest genetic diversity while Schisandra grandiflora has the lowest genetic diversity. The coefficient of genetic differentiation (Gst=0.2383) reveals that the genetic variation of the four species of Schisandra Michx. plants in Qinling Mountains was mainly intraspecific genetic variation, accounting for76.17%.6. Four species of Schisandra Michx. plants in Qinling Mountains can be divided into two categories according to the UPGMA clustering graph based on the genetic similarity coefficient of0.62. The first type includes Schisandra sphenanthera, Schisandra grandiflora and Schisandra rubriflora and the second includes Schisandra propinqua var. sinensis while the genetic similarity coefficient of0.65four species of Schisandra Michx. plants can be divided into four categories.7. The consistency of four species of Schisandra Michx. plants in Qinling Mountains genetic diversity analysis results by RAPD and ISSR was detected by Mantel detection, the results showed positive correlation and the difference is not significant. Comparison of two kinds of markers can reflect the efficiency of the percentage of polymorphic bands (PPB), resolving power of the primer (RP), the effective multiplex ratio (EMR) and Polymorphism index (PI), the results show that the RP (8.86) and PI (7.93) of ISSR marker was higher than that of RAPD markers (RP=8.50, PI=4.15), Mantel detected two labelling efficiency parameters were correlated and the difference is not significant. The results showed that both RAPD and ISSR can be used for four species of Schisandra Michx. plants in Qinling Mountains genetic diversity analysis, and the ISSR technology is more stable than RAPD.