| Objective To study the mechanism of SP in electroacupuncture(EA) intervention on neuropathic pain by observing effect of electroacupuncture on Pain Behavior Responses and substance P level in plasma and corresponding segment of spinal cord in rat with neuropathic pain.Methods Choosed to use an animal model of spared nerve injury (SNI), Some aged female rats were randomly divided into blank control group, sham operation group, model group, EA group and Sham EA group. Rats in EA group were acupunctured in Huantiao and Weizhong, lasted for 30 minutes once a day for 7 days. Then, Radiommunoassy was used to determine the levels of SP in plasma and corresponding segment of spinal cord in all rats, observed pain behavior responses and statistical analysis was done.Results 1. After the setting up of a model, paw withdrawal mechanical latency was decreased significantly as compared with before (P＜0.01) 2. After electroacupuncture intervention, paw withdrawal mechanical latency in EA group was increased significantly as compared with model group and sham EA group(P＜0.05), but there was no significant difference between EA group and blank control group(P＞0.05) 3. paw withdrawal thermal latency had no significant change in each group(P＞0.05) 4. The peripheral blood SP had no significant change in each group (P＞0.05) 5. SP level in corresponding segment of spinal cord in EA group was obviously higher than other groups, (P＜0.05). SP level in corresponding segment of spinal cord in model group was lower than blank control group(P＜0.05), but there was no significant difference between model group and sham EA group(P＞0.05), No significant difference was occurred between blank control group and sham operation group(P＞0.05)Conclusion Electroacupuncture has an obvious analgesic effect in a rat model of neuropathic pain, the mechanism might be that EA could increase SP level in corresponding segment of spinal cord by reducing the release of SP caused by a rat model of neuropathic pain. |
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