Arsenic Levels Of Ecological Environment And Toxicity Of Human RBC Membrane In Endemic Arsenicosis Area

[Objective]1. To investigate the arsenic levels of vegetables and fruits and discuss the potentialdangerous from intaking of arsenic through the food chain in Datong city of Shanxi province.2. To discuss the effect of the erythrocyte membrane protein by low levels arsenic exposure.[Methods]Eighty-five inhabitants from one village in endemic arsenism area in Datong city, Shanxiprovince were collected as exposed group,and a adjacent regional economic level of a village50residents as control group. Arsenic poisoning patients diagnosis: The People’s Republic of Chinahealth industry standard for endemic arsenism was used to identify and diagnosis arsenicpoisoning. Daily drinking water and soil were collected and detected by atomic fluorescencespectrometry. The content of vegetables and fruits were detected by inductively coupled plasmamass spectrometry(ICP-MS).Extract the control group and exposed group the RBC membraneproteins, to analyse the changes of the RBC membrane proteins with the method of SDS-PAGE.[Results]1The level of arsenic in external environment1.1The concentration of water arsenic in control group is0.41±0.22μg/L, and that ofexposure group is43.92±24.01μg/L, the difference was statistically significant (P<0.05).1.2The mean value of the As acquisition of fruits and vegetables in the control group is0.0248mg/kg, and10.00%of samples were higher than the maximal permissible limit of As infood. The mean value of As the acquisition of fruits and vegetables in the exposure group is0.0598mg/kg, and43.03%of samples were higher than the maximal permissible limit of As infood.1.3The results of the arsenic concentration of vegetables constant changes in the leafvegetables>tubers>fruit vegetables.1.4The health risk of intaking arsenic polluted vegetables constant changes in the leafvegetables>tubers>fruit vegetables. The health risk of the leaf vegetables up to71.77%.1.5The levels of water and vegetables of43.88μg/L and0.0598mg/kg would equate to69.71%and10.04%of the MTDI.1.6Arsenic content of collected soil of control group range of5.671~7.037mg/kg, acquisition of soil arsenic exposure group range of6.187~11.26mg/kg, both of them have notbeen exceeded the national standard (GB15618-1995, level of15mg/kg).2the change of the erythrocyte membrane proteinsseven different proteins, including spectrin-α, spectrin-β, ankyrin, band3protein, band4.1protein, band4.2protein and actin, were isolated from the erythrocyte membrane of men bySDS-PAGE. Compared with the control group, the differences of ankyrin,band3have statisticalsignificance (P <0.05),other differences have no statistical significance (P>0.05).The majorprotein content of the erythrocyte membrane, red blood cell count and hemoglobin content wereincreased, there have statistical significance (P <0.05).[Conclusions]1.Water→plants→human may be another way exposed to arsenic of local residents.2. Peripheral blood erythrocyte might be the targets of arsenic action in endemic arsenicosisarsea.The change of the erythrocyte membrane proteins may be the early biological markers ofchronic arsenic poisoning.