Study On Sperm DNA Damage And Aneuploidy Sperm In Subfertile Men

Objective: Use Sperm chromatin dispersion (SCD) test analysis the level of DNAdamage frequency and Fluorescence in situ hybridization (FISH) test measure thechromosomal(18,X,Y) aneuploidy ratio, To investigate the changes in sperm DNAintegrity and chromosomal abnormalities in some subfertile patients and its clinicalsignificance.Methods：Semen samples were obtained from healthy normal and subfertile men. Allsamples were classified as normal group and subfertile groups(Oligozoospermia,Asthenozoospermia and Oligoasthenozoospermia) according to World HealthOrganization guidelines. The level of DNA damage frequency was determined bySperm chromatin dispersion (SCD) test and the chromosomal(18,X,Y) aneuploidy ratiowas analyzed by Fluorescence in situ hybridization (FISH).Results：Significant DNA damage frequency differences were found among controlgroup and subfertile groups (P＜0.001); And aslo significant differences were noted insperm chromosomal(18,X,Y) aneuploidy ratio between control group and subfertilegroups (P＜0.05);There were significant positive correlations between Sperm DNAdamage frequency and sperm chromosomal aneuploidy ratio (r=0.453,P=0.008).Conclusion：In infertility patients sperm DNA damage frequency and chromosomeaneuploidy ratio are obviously higher than normal control group and has significantpositive correlation. In the process of meiosis, DNA damage may be the risk factorcausing sperm chromosomal aneuploidy.