The Expression Of Foxp3and CD8in Adenocarcinoma Of Esophagogastric Junction (AEG) And Their Clinical And Prognosis Meaning

Objective To explore the expression of regulatory T cell (Treg) and CD8+T cell intissues of adenocarcinoma of esophagogastric junction (AEG), and analyze theircorrelation with carcinogenesis. tumor progression and postsurgical5-year survival..Methods To collect Department of General Surgery of Anhui Medical University,January2004-December2006data of68cases of patients with adenocarcinoma ofesophagogastric junction, including49males and19females, age41-76(median59)years old, specimens were neutral4%formaldehyde. Using the2010InternationalUnion Against Cancer/American Joint Committee on Cancer (UICC/AJCC) TNMstaging criteria, Ⅰ, Ⅱ of28cases of stage Ⅲ40cases; high grade23cases,45casesof poorly differentiated; Swerit type Ⅱ23cases, Ⅲ45cases. with20peptic ulcertissues selected as control. Each case has detailed clinical data, surgical records,pathology, and more than5years of follow-up results. All patients were not related tothe treatment of chemotherapy-line release, underwent R0resection of the tumor, allcases petitioners dated December20,2011. We dyed the tissues usingimmunohistochemistry, evaluating the expression of CD8+T cell with Foxp3and Tregsas markers, and studying their relationship with TNM classification, differentiateddegree, lymphatic metastasis, tumor location., and postsurgical5-year survivalResults The positive rate of Foxp3and CD8+T cell in AEG group was significantlyhigher than that in control group (P<0.05). the distribution of Foxp3+in experimentalgroup with lymph node metastasis was higher than that without lymph node metastasis(P <0.05),,the positive rate of poorly differentiated was significantly higher than high-differentiated group on Foxp3(P <0.01), and TNM stage late (Ⅲ) group wassignificantly higher than earlier TNM stage (Ⅰ~Ⅱ) group (P=0.001)The expression of Foxp3is closely relate with the clinical stages,grades of the masses,the rate oflymphatic metastasis, and significantly higher in group with lymphaticmetastasis,poorer differentiation, later stage,while not significantly correlated with theposition,age and gender of the patients(P>0.05). Foxp3was negatively correlated withCD8in malignant tissues (r=-0.314, P=0.01). Foxp3positive distribution rate ofpostoperative survival over five years not exceeding five years less than the cases, thedifference was statistically significant (P <0.05)Coclusion The expression of Foxp3+Treg is significantly associated with AEGcarcinogenesis and progression. Tregs in AEG tissues possibly promote tumorprogression via inhibiting function of CD8+T cells.