The Effects Of P38MAPK Signaling Pathway Inhibitor SB203580on Hypoxia-reperfusion Of Liver Cells

Objective To investigate the effects of P38MAPK inhibitor SB203580on cellgrowth,migration,invasion and apoptosis on hepatoma cell line HepG2,and cellgrowth,apoptosis on normal human cell lines LO2of hypoxia-reperfusion process.Methods HepG2cells and LO2cells were treated with different concentration ofSB203580and different hypoxia time respectively,to determination the proliferation ofHepG2cells and LO2cells by MTT.The HepG2cells and LO2cells were treated withSB203580at the dose of0.5μmol/Land0.1μmol/L,Annexin V-FITC/PI assay to detectthe cells apoptosis.And then,detected the P38protein expression by WesternBlot.Wound healing test and invasion assay was applied to measure the change ofmigration and invasive ability respectively.Results Compared with normal control group,the HepG2cells apoptosis rate andP38MAPK phosphorylation levels of the hypoxia group were increased,while comparedwith hypoxia control group,the HepG2cells apoptosis rate was increased andP38MAPK phosphorylation levels was decreased of the SB203580treatment hypoxiagroup;After48h in scratch test,the control group of HepG2cells moved to the center ofthe scratch,and SB203580treatment hypoxia group was inhibited;After24h inmigration assay, SB203580treatment hypoxia group of HepG2cells’invasive abilitywas inhibited compared with the control group (p<0.05);In Annexin V-FITC/PI assay,the cell apoptosis rates of the SB203580treatment hypoxia group showed a significant increase in HepG2cells and decrease in LO2cells when compared with control group(p<0.01)；In MTT assay,SB203580affected the HepG2cells and LO2cells’proliferationinhibition rate,and depended on the time and concentration.Conclusion SB203580through blocking P38MAPK signal transduction pathwayspecifically,have a protective effect on LO2cells and promote the apoptosis,inhibit theproliferation and migration,reduce invasion capabilities on HepG2cells.