Research On Distribution And Drug Resistance Of Pathogen Cultured In A General Hospital From2011to2013

ObjectiveTo analyze and the distribution and drug resistance of the pathogens cultured from the submitted specimens from a general hospital in Liaocheng and explore the drug resistant mechanisms so as to guide the reasonable medication.MethodsThe source of the culture specimens, distribution of pathogens, and the drug resistance were taken for statistical analysis from2011to2013. Bacteria identification and drug sensitivity tests were conducted by VITEK-2Compact automatic identification method and other supportive tests. The data was collected by WHONET5.4, and the clinical distribution and drug resistance of the strains were analyzed by SPSS17.0.ResultsFrom2011to2013, the number of pathogens we separated increased year by year, altogether there were13277non-repetitive isolates. Among these specimens, the number of respiratory specimens was62.8%, followed by blood,13.3%. The major pathogenic strains we separated during the three years were gram-negative bacteria. In2011, we separated3913strains in total, with2944in G-(75.23%),926in G+(23.66%),43in fungi (1.10%). In2012, we cultivated4493strains, with3310in G"(73.67%),1111in G+(24.72%),72in fungi (1.60%). In2013, we fostered4871strains, with3483in G+(71.50%),1278in G+(23.66%),110in fungi (2.26%). There was no apparent variation in the distribution of bacteria. ICU separated the most pathogens clinically, at12.96%, followed by Respiration Medicine.In2011, the strains we separated were Pseudomonas aeruginosa (20.01%), Staphylococcus aurous (19.00%), Acinetobacter baumannii (17.20%), Klebsiella pneumonia (16.28%) and Escherichia coli (14.52%). In2012, we isolated Escherichia coli (21.01%), Acinetobacter baumannii (13.55%), Pseudomonas aeruginosa (13.39%), Klebsiella pneumonia (10.75%) and Staphylococcus aureus (10.15%). In2013, our achievements were Escherichia coli (26.59%), Pseudomonas aeruginosa (11.85%), Klebsiella pneumoniae (10.24%), Staphylococcus aureus (10.12%) and Acinetobacter baumannii (8.70%) were the top five. During the three years, the top5pathogens haven’t changed much. Gram-negative bacteria were the dominant bacteria and remained over70%throughout the three years. The drug resistance of the separated pathogens were as follows:Gram-negative bacteria showed resistance to different extents to broad-spectrum penicillins (such as Ampicillin and Amoxicillin), Quinolones (such as Cephalosporin, Ciprofloxacin and Ofloxacin) within four generations and Aminoglycosides (such as Gentamicin, Tobramycin and amikacin). Staphylococcus aureus resisted over80%of Penicillin, Erythromycin and Clindamycin, but the rate fell in resistance to Oxacillin year by year. Enterobacteria were still the most sensitive to Carbapenems with a sensitive rate over98.6%. However, the emergence of Carbapenem-resistent enterobacteria in the three years is worthy of clinical attention.