A Clinical Data Analysis Of MiR-34a Expression In Borle Marrow T Cells Of Aplastic Anemia

Backgrounds and ObjectivesAplastic anemia(AA), characterized by pancytopenia, is a paradigm of the human bone marrow failure syndromes. The pathophysiology of AA is mostly immune mediated, with activated type1cytotoxic T cells implicated. AA can now be cured or ameliorated by stem-cell transplantation or immunosuppressive drug therapy. MicroRNA (miRNA)s are a family of evolutionarily conserved small non-coding RNAs regulating gene expression by posttranscriptional gene repression or mRNA degradation, which have been found to control cell division, differentiation, death and normal immune function. To date, various miRNAs have been found abnormally expressed in immune-related diseases such as ITP(immune thrombocytopenia), RA(retinoid arthritis) and SLE(systemic lupus erythematosus). MiRNA chip result found that miR-34a was expressed significantly higher in AA patients bone marrow T cells than those in normal controls. This research is to detect the expression pattern of miR-34a in bone marrow T cells of different types of AA patients.MethodsBone marrow T cells were isolated from31AA patients and14healthy controls, whose miR-34a expressions were detected using quantitative real time RT-PCR. The relationship between sex, age, AA severity, NEU number, RET number, PLT number and miR-34a level was analyzed respectively using SPSS17.0.Results1. No significant differences of miR-34a level were detected in AA patients bone marrow T cells with different sexes(P=0.5109) or ages(P=0.1565).2. Significantly higher miR-34a level was detected in AA patients bone marrow T cells than in normal controls(p<0.0001), showing a positive correlation with AA severity(r=0.451,p=0.011).3. MiR-34a level in AA patients bone marrow T cells was in a negative correlation with NEU number(r=-0.4279,p=0.0163) and RET number(r=-0.3603,P=0.0465), while uncorrelated with PLT number(r=-0.2005,P=0.2794).ConclusionsOur results show that miR-34a level in AA patients bone marrow T cells was significantly higher than in normal controls, which was with a positive correlation with AA severity and a negative correlation with NEU number and RET number, but was uncorrelated with sex, age and PLT number. This is instructive for laboratory diagnosis, typing, clinical therapy choice and prognosis in AA.