The Study Of Green Fluorescent Protein As Marker Protein In The Expression Of Insulin

Objective To investigate the correlation of fluorescence intensity ofEGFP and expression of insulin.Methods Construct recombinant plasmid prIns1EGFP and transfectHIT-T15cells, the cells were cultured and randomly divided into three groups,low glucose group(LG)、 middle glucose group(MG) and high glucosegroup(HG),The released insulin and A single islet beta cell relative fluorescenceintensity at baseline and after4hours stimulation were measured bychemiluminescence analysis(CA) and fluorescence microscope methodrespectively, protein level was detected with immunocytochemistry.Results The level of insulin and a single islet beta cell relativefluorescence intensity on the HG groups was higher than LG group（P<0.05）,theexpression level of proinsulin on the HG group was higher than LG group （P<0.05）,after the glucose stimulation,, there was significant change of asingle islet beta cell relative fluorescence intensity in HG group（P<0.05）,withina certain range of glucose concentration, compared fluorescence intensity andprotein level at the corresponding time shows that they have positive correlation.(r=0.896)（P<0.05）.Conclusions The fluorescence intensity of EGFP and level of insulinhave good correlation.