FSTL1Impact On Immune Function Of Dendritic Cells In Patients With Nasopharyngeal Carcinoma

Objective：This experiment aims to research follistatin-like protein1(FSTL1) stimulate the mature dendritic cells (DC) of patients withnasopharyngeal carcinoma. And then investigate the impact of the DC’s immunefunction on FSTL1. Explore the potential about FSTL1as cancerimmunotherapy drugs.Methods： Collected peripheral blood25ml from17nasopharyngealcarcinoma patients and10healthy volunteers, and isolated mononuclear cells(PBMC). Separated monocytes and lymphocytes by adherent. There wereHLA-A2+monocytes of8NPC patients and6healthy volunteers screened byflow then continue cultured. Lymphocytes were cryopreserved if used insometimes. Monocytes were induced to mature DC by rhGM-CSF (1000U/ml),rhIL-4(500U/ml) and rhTNF-α (1000U/ml), and phenotype include CD40, CD86and HLA-DR were detected in the first three days and eight days.Collected mature DC and divided into two groups: the first group received notreatment, scilicet DC group; the second group joined FSTL1as stimulus,scilicet DC+FSTL1group. Two groups of DC presented antigenic peptides FLY,and then DC and autologous lymphocyte by recovery were blended cultured.Cells were collected in the first fifteen days. Detected DC antigen-presentingand stimulated lymphocytes turn into specific lymphocytes by Elispot andTetramer.Results：1．We successfully induced maturation of dendritic cells, irregular shapeand many different lengths of the projections, the form was dendritic.2．Elispot results:①Nasopharyngeal carcinoma patients group: The specific lymphocytecounts (scilicet number of spots) which secreted IFN-γ of DC group and DC+FSTL1group medians were40.00(11.00-93.00),51.00(31.00-118.00). Thedifferences were statistically significant (P <0.05);②normal control group:The specific lymphocyte counts (scilicet number of spots) which secreted IFN-γof DC group and DC+FSTL1group medians were59.50(34.33-88.67),72.00(41.00-104.00). The differences were statistically significant (P <0.05);③DC+FSTL1group between NPC patients and normal control were compared andthe differences were not statistically significant (P>0.05). 3．Tetramer results:①Nasopharyngeal carcinoma patients group: The specific lymphocytecounts(%) of DC group and DC+FSTL1group medians were0.86(0.15-2.09),1.38(0.41-3.82). The differences were statistically significant (P<0.05);②normal control groups: The specific lymphocyte counts(%) of DC group and DC+FSTL1group medians were1.75(0.13-2.60),2.63(0.23-3.75). Thedifferences were statistically significant (P<0.05);③DC+FSTL1groupbetween NPC patients and normal control were compared and the differenceswere not statistically significant (P>0.05).Conclusion：FSTL1could improve immune function of dendritic cells innasopharyngeal carcinoma patients.