Effects Of Resveratrol On High Glucose-induced Injury To Endothelial Cell

Objective:To explore the effects of resveratrol on high glucose-induced endothelial cell injury.Methods:Cultured human umbilical vein endothelial cells(HUVECs) were divided into6groups:normal control group(NG), mannitol control group(MA), high glucose group(HG), low dose resveratrol group(LR), medium dose resveratrol group(MR), high dose resveratrol group(HR). HUVECs were pretreated with or without30mM glucose plus various concentrations of resveratrol(0.1,1,10μ mol/L) for16hours and then treated them with5.5mM glucose for6days. The levels of vascular cell adhesion molecule1(VCAM-1), monocyte chemotactic protein1(MCP-1), and plasminogen activator inhibitor-1(PAI-1) in the culture supernatant were measured by means of ELISA. NFκB expression was detected by Western blot.Results:Compared with NG group, hyperglycemia memory up-regulated the expression of NFkB, along with the increased the levels of VCAM-1, MCP-1and PAI-1(all P<0.05), which were still maintained at high levels after withdrawal of high glucose. Resveratrol treatment down-regulated the expression of NFκB and lowered the levels of VCAM-1, MCP-1and PAI-1. The effects were in a resveratrol dose dependent manner. There was no significant difference in the expression of NFκB, VCAM-1, MCP-1and PAI-1between mannitol group and normal group.Conclusion:Resveratrol may decrease the secretion of VCAM-1, MCP-1and PAI-1and prevent high glucose memory-induced injury to endothelial cell via NFκB pathway.