Association Of Gene Mutation At Position-29in Promoter Of Follicle-stimulating Hormone Receptor With Ovarian Response

Objective To explore if the genetic variant at position-29(Gâ†'A) in promoter of Follicle-stimulating Hormone Receptor (FSHR) in peripheral blood leukocytes of patients is associated with ovarian responses in in vitro fertilization and embryo transfer (IVF-ET) by measuring the genotype of position-29(Gâ†'A) in promoter.Methods We divided85women who were undergoing IVF-ET into three groups according to ovarian responses receiving controlled ovarian hyperstimulation(COH). They are over response group with20patients, normal response with46patients and poor response with19patients. Polymerase chain reaction-restriction fragment length polymorphism was used to examine the gene polymorphism of the FSHR promoter.Resultsâ‘ The mutative rate of poor response group is42.1%; the rate of normal response group is10.87%; and the rate of over response group is10%.â‘¡The mutative rate of poor response group is higher than that of groups of normal response and over response (P<0.05). There is no significant difference between the normal group and over group (P>0.05)â‘¢The serum concentration of estradiol (E2) on the days of HCG injection and the median numbers of oocytes retrieved per patient in poor response group are lower than that in other two groups (P<0.05). And the serum concentration of E2on the days of HCG injection and the median numbers of oocytes retrieved per patient in normal response group are lower than that in over response group (P<0.05)Conclusion The gene mutation at position-29(Gâ†'A) rate of the FSHR promoter in peripheral blood leukocytes may is related to ovarian responses. It may be used as a marker to predict ovarian responses in IVF-ET.