Expression Of Transforming Growth Factor Beta1of Cryopreserved Skin Transplantation

BACKGROUND:Skin grafting and wound coverage are the most important measures for the treatment of wound and burn. Auto-skin grafting would make the secondary damage of normal tissues hurt. And allogenic skin grafting has two problems about immunological rejection and the long-term preservation of skin. Cryopreservation is expected to improve these limitations.OBJECTIVE:To evaluate the application of allogeneic skin in the wound by detecting the expression of transforming growth factor-β1in rat skin transplantation under cryopreservation.METHODS:Forty-two SD rats were randomly divided into three groups by random number table method:hypothermic preservation (-20℃) group (N=18), cryopreservation (-80℃) group (N=18) and autograft group (N=6). Experimental groups were divided into three subgroups respectively:The skin specimens of rats were preserved for1week,1month and2months. Then skin pieces in each group were transplanted into the back of rats. The control group made autologous skin graft. Taking samples of the skin to detection at Day3, Day7, Day10and Day20.RESULTS:The baseline of different groups was comparability. Compared with preservation group, rejection of skin transplantation in cryopreservation group was delayed and survival time was prolonged, the expression of transforming growth factor-β1was inhibited. It was said that the cryopreservation could reduce the immunological rejection.CONCLUSION:The results show that cryopreserved transplantation skin as a kind of wound temporary coverage can reduce the immunological rejection. And it has s wide application prospect in case that skin tissue is inadequate or defection.