Effects Of BMP-7on Snaill Expression And Epithelial-Mesenchymal Transition In Podocytes Induced By High Glucose

Objective To investigate the effect of BMP-7on snaill expression and epithelial-mesenchymal transition (EMT) induced by high glucose in vitro and to explore the possible mechanisms of BMP-7inhibition of podocyte EMT in diabetic kidney disease (DKD)Methods Cultured mice podocytes were coincubated with high glucose for different periods of time(6、12、24、48hours), then were randomly divided into four groups:normal glucose group (NG), high glucose group (HG), high glucose+BMP-7group (BMP-7) and mannitol control group (MG). The cell morphological changes were traced with inverted microscope. The mRNA expressions of snaill、nephrin and a-SMA in podocyte were detected by real-time quantitative RT-PCR and the protein expressions of p-smad2/3、snaill、nephrin and a-SMA were detected by westernblot.Results After treatment with high glucose, The podocytes converted from typical arborized cells into cobblestone appearance, and the expressions of snaill and a-SMA were up-regulated considerably while the expression of nephrin was significantly decreased in a time-dependent way (P<0.05). BMP-7alleviated the up-regulation of p-smad2/3、snaill and a-SMA expressions and down-regulation of nephrin expression in podocytes induced by high glucose to some extend (P<0.05). There were no significant differences between NG group and MG group (P>0.05).Conclusions High glucose can up-regulate snaill expression and induce EMT in podocyte in a time-dependent way and BMP-7can inhibit podocyte EMT induced by high glucose possibly by regulating TGFβ/smad pathway and the expression of snaill.