| Objective:To analyze the expression of TLR4, NF-κB and IL-6after brain injury in rats.Methods:64adult male SD rats,250g-350g, were randomly divided into3groups:Normal group(NC group)(8rats) which were not given any treatment. Operation group(SO group)(8rats) which given general anesthesia and craniotomy without a shock. Brain Injury group(BI group)(48rats) which were given general anesthesia and a shock.At1hour,6hour,12hour,24hour,3day and7day after being shocked with BI group, After anesthesia by10%chloral hydrate, brains were taken out and the damage area of brain tissue was taken out3, for detection of TLR4, NF-κ B, IL-6by Western blot.Results:1Expression of TLR4In BI group, TLR4expression began increase at6th hour(1.49±0.55, P<0.05), increase at12th hour(3.61±0.48, P<0.05), and reached its peak at24th hour(5.60±1.60, P<0.05), being significantly higher than NC group (P<0.05).7th day, TLR4expression decreased (2.34±0.53, P<0.05).2Expression of NF-κBIn BI group, NF-κB expression began to increase at6th hour (4.48±1.20, P<0.05), and reached peak at24th hour (8.50±1.55), being higher than NC group (P<0.05). At7th day, NF-κB expression decreased (2.56±0.96, P<0.05).3Expression of IL-6BI group compared with NC group, at6th hour, the IL-6expression ascended slightly (3.65±0.38, P<0.05), but rosed significantly higher to12th hour (4.95±1.05, P<0.05), up to from24th hour to3rd day(7.75±1.64, P<0.05), at3rd day it becamed lower (3.35±0.75, P<0.05).Conclusions:1The expression of TLR4, NF-κB and IL-6, was increased trend after fluid percussion injury in rats.2TLR4, NF-κB and with IL-6, expression were positively correlated by fluid percussion brain injury with the rat.3To control critical link of TLR4-NF-κB pathway, the intracellular signal transduction pathway activity was reduced, prevent and treat inflammatory response of traumatic brain injury may be a new method. |
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