Study Of Cell Light Scattering Speckles Based On Mie Theory

Flow cytometry is usually used to distinguish and classify different kinds of cells after the cells fluorescent stained. Flow cytometry is expensive and need to be used by professional workers. More and more researchers are studying new methods in cell sorting without fluorescent stained. Light scattering is related with cell’s physical properties and its surrounding environment; it may be used to classify different kinds of cells non-contact and non-destruction. The study of light scattering to classify different kinds of cells label-free is supposed to be useful as a cell diagnostic technique.Two-dimensional (2D) light scattering technology is a new method to sort cells label-free. The Finite difference time domain (FDTD) method is used in studying2D light scattering speckle patterns in general, and this method is usually need to be parallel computed. We study a new method to simulate2D light scattering speckle patterns of single cell based on Mie theory, this method will significantly reduce the requirements in computational simulation performance and promote further understanding of2D light scattering speckle patterns.The background knowledge about light scattering of microscopic particle is introduced firstly and the electromagnetism characteristic, phenomenon and theory of light are mainly presented. The2D light scattering patterns of single cell with only one organelle is simulated after introducing the basic knowledge of the cell and Mie theory briefly. The effects of the size and relative refractive index of organelle on simulated light scattering patterns are discussed. Finally the light scattering speckles of a single cell with multiple organelles are discussed. The way to calculate multiple organelles are studied at beginning. The effects of the size and relative position of the organelles on simulating scattering patterns of the single cell are discussed and how different combinations of the organelles can change the2D simulating light scatting patterns are also discussed.