Expression And Significance Of PRX2, AKR1B10, E-FABP And67LR During Two-stage Model Of Chemically Induced Hepatocellular Carcinoma In Mice

Objective To explore the expression and significance of PRX2,AKR1B10,E-FABP and67LR during two-stage model of chemically inducedhepatocellular carcinoma in mice.Methods Sixty C57BL/6J male mice were induced to establish primaryhepatocellular carcinoma (HCC) models by combination of diethylnitrosamines(DEN), carbon tetrachloride (CCl4) and ethanol, the other30C57BL/6J micewere included as normal control group(no drug was given). Mice weresubsequently sacrificed at every4weeks after indicated times, remaininganimals were sacrificed at the end of the study (20weeks). Liver tissue wascollected for pathological analysis with hematoxylin-eosin (H-E) stain;at thesame time, Detected the variance of PRX2,AKR1B10,E-FABP and67LR anmRNA and protein level by FQ-RT-PCR and IHC,respectly.Results First of all,The control group was normal liver tissue, liver cellsarranged radially central vein, normal lobular architecture;The experimental group successfully induced liver neoplasms in mice.In experimental group, toxichepatitis and acute hepatocellular necrosis were observered at4th week; thehepatocellular necrosis got worse and liver fibrosis were observered at8th week;then, atypical hyperplasia, pseudolobule and hepatocellular carcinoma wereobservered at12th,16th,20th week respectively.Next,Firstly,The results ofFQ-RT-PCR and IHC showed that the expression levels of PRX2and AKR1B10in experimental group were significantly higher than that in normal controlgroup from4th to20th weeks (P<0.05) and the expression levels of E-FABP and67LR in experimental group were significantly higher than that in control groupfrom8th to20th weeks (P<0.05).Secondly,in experimental group, the expressionlevels of PRX2,AKR1B10,E-FABP and67LR kept on rising as time progressed,in addition to the individual time points, the expression between the stages ofcarcinogenesis time points were different, and the difference was statisticallysignificant (P<0.05);Thirdly,In20weeks,experimental group was significantlyhigher than precancerous experimental group (P<0.05);Fourthly,The proteinexpression of PRX2,AKR1B10,E-FABP and67LR was localized in thecytoplasm.Conclusions The overexperession of PRX2,AKR1B10,E-FABP and67LRmay be the early events during chemical-induced hepatocellular carcinoma inmice and may promote the startup of liver neoplasms and hepatocarcinogenesis.