Fingolimode Inhibits Proliferation Induced By S1P1/S1P3Pathway In Rat Carotid Artery After Balloon Injury

Objective:To observe the expression of Sphingosinel-phosphate receptor1/3(S1P1/S1P3) in rat carotid artery after balloon injury and effection of fingolimode on inhibiting restenosis.Methods:Sixty SD rats were equally and randomly divided into the sham operation group, the negative control group, the balloon injury group and the drug intervention group. Carotid arteries of rat were extracted at3d,7d and21d, the vessels were stained by hematoxylin-eosin to observe the proliferation. The expression of S1Pl and S1P3in target vessels was assessed by western blot.Results:Compared with other group, the proliferation of the balloon injury group was significant. The expression of AKT2mRNA in drug intervention group was lower than the balloon injury group, but there was significance different between those two groups at7d (P<0.05).The quantity in the drug intervention group and balloon injury group was higher than the blank group and the negative control group (P<0.05).There was no significance different between sham operation group and the negative control group (P>0.05).Western blot showed that at the initial of the balloon injury the expression of S1P1and S1P3was in the high level. Compared with7d the expression of S1P1and S1P3was in the lower level in21d, especially after treated with fingolimode.Conclusion:S1P1and S1P3play the important role in migration and proliferation of vascular smooth muscle cells, Fingolimode can attenuate the restenosis by inhibiting the expression of AKT2, S1P1and S1P3.