The Anticancer Effects Of Curcumin On Human Breast Cancer And Its Possible Mechanism

Breast cancer is the most common form of cancer in women，and it also caused aserious threat to the women’s health. The breast cancer is more prone to metastasis.Then, a lot of researchers dedicated to the research on the related mechanism.Although there are quite a large number of factors can be used, but it is still necessaryto develop new anticancer drugs and apply to clinical practice.MiRNAs are a new type of small RNA molecules. miR-21is a new cancerresearch focus in the recent development. It changed associated proteins throughregulated related gene expression. Meanwhile, it also plays an important role involvedtumor gene. It play a similar cancerigenic role.Turmeric is a activity ingredient in Turmeric. Studies have shown that curcumincan resist proliferation and invasion of breast cancer. and the anticancer mechanismof curcumin also have reported. But for explained its’ anticancer mechanism,curcumin is associated with miR-21has not been reported.Objective:In this study, MCF-7as cell model. Investigate curcumin to the proliferationand the related miR-21gene expression for this cell lines. Make further efforts toexplore the curcumin resist related signaling pathways and molecular mechanisms ofbreast cancer. Provide theoretical basis for clinical application.Method:1.Detect the viability of cell lines by means of MTT assay. The experiment wasperformed1group normal control and5experimental groups. Use curcuminconcentration of2.5μM、5μM、10μM、20μM、40μM treated cells respectively.Investigate the effect of curcumin to the cell.2.In order to detect apoptosis, use caspase-3kit to experiment. Divide into groups andcurcumin ‘s concentration is the same as above.3.Observe cell cycle of MCF-7use FCM. Divide into groups and curcumin′s concentration is the same as above.4. Detect the expression of miR-21through real time PCR. Resercher on the influenceof curcumin.5. Detect the expression of PDCD4that is miR-21’s target genes by immunoblotting.Result:1. The results show that curcumin strengthen killing effect to the cell and weaken cellviability as increasing concentration.2. After treatment24hours, the results showed that OD values increased significantlyand caspase-3’s activity increased with increasing concentrations of curcumin.Meanwhile,it activated apoptotic pathway and induced apoptosis of breast cancercells.3. After treatment24hours, the results showed that cell cycle changed significantly.The results showed that G2phase cells increased significantly (P <0.05).4. The results showed that the expression of the miR-21reduced gradually with thetreated concentration of curcumin from0μM,2.5μM,5μM,10μM,20μM respectively.Compared with the untreated group, the expression of miR-21decreased significantly(P<0.01).5. The results showed that the expression of the miR-21increased gradually with thetreated concentration of curcumin from0μM,2.5μM,5μM,10μM,20μM respectively.It showed that the maximum expression level in the20μM.In conclusion, the expression of miR-21decreased with the curcumin’sconcentration rising. This also triggered the expression of PDCD4increased.Thereby,it may inhibit the Ras-Ap-1pathway that is cancerigenic pathway andpromote apoptosis of tumor cells. This provides a theoretical basis that molecularmechanism about the anticancer effects of curcumin.Conclusion:1. Curcumin can inhibit the proliferation of MCF-7breast cancer cells in vitro. Andwith increasing concentrations, inhibit the role strengthened.2. Curcumin concentration increases, cells reduces the amount of miR-21expression,protein expression of downstream target genes PDCD4elevated, inhibited Ras-AP-1 signaling pathways.3. Curcumin reduced miR-21and increases PDCD4expression, it may inhibit one ofthe mechanisms about proliferation of breast cancer MCF-7.