The Correlation Analysis Beetween The Elderly Unstable Angina Pectoris And Intestinal Flora Imbalance

Objective: By testing intestinal flora, containing Escherichia coli、Bacteroides thetaiotaomicron、 Lactobacillus of the elderly unstableangina pectoris and normal person, we can analyse the correlationanalysis between the elderly unstable angina pectoris and intestinal floraimbalance, and probed into the mechanism of intestinal flora imbalancewhich promoted the occurrence of unstable angina pectoris.Methods: This analysis included30patients with unstable angina and30control subjects of similar age, gender and BMI from June2013toJanuary2014,at cadre ward of First Hospital of Jilin University. Feces ofparticipants were collected0.5g of wet weight, After repeatedcentrifugation, washing. Total bacterial DNA was extracted by DNA stoolkit according to the manufacture’s protocol. Blood samples of emptystomach were collected in the very morning, testing myocardial injurymarkers, blood lipid, determination of homocysteine. Then we collectedelectrocardiography before and after the hospital. According to thepublished NCBI, the complete genome sequence of Escherichia coli、Bacteroides thetaiotaomicron、Lactobacillus was designed by primer3.0.The primers were compared in the Basic Local Alignment Search Tool(BLAST). The normal group high positive method were conducted to manufacture the standard curves. The concentration quality ofstandardand was determined by agarose gel electrophoresis. Standard wasextracted by Agarose Gel DNA Purification Kit, then was tested byUltraviolet spectrophotometer, calculating the standard concentrationaccording to the OD260.Applicating of SYBR Green I real-timequantitative PCR，each fecal sample was performed with negative control,standard. Standard curves were constructed using LightCycler480Software.Copy numbers of bacteria in fecal samples were calculated fromthe threshold cycle values (Ct) and expressed as quantity of bacteria pergram feces. Statistical analyses were performed using SPSS17.0. Theconcentration’s comparisons of Escherichia coli、 Bacteroidesthetaiotaomicron、Lactobacillus between unstable angina pectoris andnormal control were done using independent samples t-tests. Thecorrelation analysis between the intestinal flora and the level of lipidmetabolism and inflammation was used based on Pearson correlationanalysis. P-values<0.05was considered statistically significant.Results: Unstable angina pectoris group （2.49E8±5.07E8） hadsignificantly greater amount of Escherichia coli than normal group（5.42E5±1.30E6）, P＜0.05; Normal group（4.49E2±9.66E2）hadsignificantly greater amount of Bacteroides thetaiotaomicron thanunstable angina pectoris group（8.14E1±2.03E2）, P＜0.05; Normal group（1.06E6±2.58E6）had significantly greater amount of Lactobacillus than unstable angina pectoris group（3.31E4±6.43E4）, P＜0.05. Therewas a significant positive correlation（r=0.394，p=0.031）between theconcentration of Escherichia Coli and the level of Hcy in the unstableangina pectoris group. There was a significant negative correlation（r=-0.524，p=0.003）between the concentration of Lactobacillus and the levelof LDL in the unstable angina pectoris group(P＜0.05).Conclusions: The results of this study indicated that the elderlyunstable angina pectoris was characterized by an increased concentrationof Escherichia coli, while an decreased concentration of Bacteroidesthetaiotaomicron and Lactobacillus. Intestinal flora imbalance promotedthe occurrence and development of unstable angina. Lactobacillus may beassociated with lipid metabolism, reducing serum LDL.