Fractures In Installment Principle Analysis And Bone Paste External Expression Of BMP-9in Rabbit Fracture Healing

Objective Access to a large number of ancient and modern literature, as to providetheoretical basis for fractures in installment principle, establish rabbit model, fracturein three phase line of HE staining and immuno his to chemical detection theexpression of BMP in fracture healing-9, from molecular biology to explore themechanism of action of tujia medicine bone paste to promote fracture healing.Method Through the theoretical study to know the detailed effect of fractures ininstallment principle, relevant literatures on the evidence, prove the necessity of thefractures in installment in clinical therapeutic principle. Then according to the datasorting, establish rabbit fracture model, from the aspects of experiment furtherdiscusses fracture stage therapeutic effect. Select60healthy rabbits, building on theleft side of the NaoGu3mm bone defect model, were randomly divided into bonepaste group (hereinafter referred to as the experimental group) and the model controlgroup (hereinafter referred to as the control group),30in each group. After beingwound dry after building experimental tujia medicine bone paste gels, and small splintfixation, control group not medication, with only small splint fixation. Respectively inthe building after the end of the3w,6w and10w to be put to death animals, eachgroup of10bone tissue specimen after paraffin embedding tissue section, line HEconventional dyeing and immuno his to chemical detection, in the third phase of BMP-9in the expression in the process of fracture healing. Using data from statisticalsoftware analysis.Results Chinese medicine fractures in installment principle guiding treatment of thefracture has become a basic guiding principle, in3,6,10weekend to be put to deathanimals, remove bone tissue specimens line HE staining and immuo his to chemical detection of the expression of BMP-9, that can be observed in the three periods offracture end group in terms of histology, fracture healing is obviously better than thecontrol group, immuno his to chemical test results, the experimental group of BMP-9expression in the three periods of bone mother cell shading condition is good, theexpression of BMP-9is higher than the control group.Conclusion Fractures in installment of TCM therapeutic principle plays an importantrole in guiding the fracture healing process, under the guidance of the basic principlesof drug use, can effectively promote the fracture healing, tujia medicine bone paste inthe treatment of traumatic fractures of the role of a verification is amenable to clinical,bone paste during early use can improve the blood circulation of the fracture end,promote angiogenesis, medium-term using bone paste can make the fracture damageto the characters, promote osteo genesis cells to differentiate into bone cells, achievethe goal of continued reinforced bone, late use of bone paste, can improve thefunction of zang-fu organs, qi is sufficient, provide material base for fracture healing,shorten the time of fracture healing. This research adopts the rabbit fracture model, setup the experimental group and the control group, the histology and immuno his tochemical through three cycles of rabbit to observe the healing of fracture, is clear thatthe experimental fracture healing of rabbit is better than control group, while theexpression of BMP-9in fracture quantity is higher than the control group, itsinducing osteogenic effect will be better than control group, BMP-9can induce andectopic bone formation, thereby promoting fracture healing.